báo cáo khoa học:" Unmet needs, quality of life and support networks of people with dementia living at home" potx

... and support for people with dementia London: The Stationery Office; 2007.37. Miranda-Castillo C, Woods B, Orrell M: People with dementia living alone:what are their needs and what kind of support ... quality of life and support networks of people with dementia living at home. Health and Quality of Life Outcomes 2010 8:132.Submit your next manuscript to BioMed Central and take full advantage of: ... toinvestigate the relationship between unmet needs, social networks and quality of life in PWD living at home aim-ing to identify factors that are associated with and/ orpredict unmet needs...

Tài liệu Báo cáo khoa học: TEC family kinases in health and disease – loss-of-function of BTK and ITK and the gain-of-function fusions ITK–SYK and BTK–SYK pptx

... redistribution of electrostatic charges that areFig. 1. Structure of PH, SH2 and kinase domains of BTK with color-ing of residues affected by missense mutations. Top left: locations of the missense mutation ... produces a very stable and shorter proteinin COS7 and 293T cells. This shorter isoform, whichcan also be phosphorylated, is generated as a result of alternative translation initiation. BTK–SYK also ... largepopulations are analyzed, will also identify individuals with mild disease, thereby providing the missing data.The phenotype of XLA and thepotential of BTK and ITK inhibitorsThe outcome of defective...

Tài liệu Báo cáo khoa học: Competition between innate multidrug resistance and intracellular binding of rhodamine dyes pdf

... centrifugation for5 min, and incubation for at least 1 h at room tempera-ture. The dissolved samples were mixed thoroughly with 0.5 mL of water and centrifuged for 5 min. Samples(0.4 mL) were withdrawn ... separated fromthe external medium by centrifugation through an oilcushion and dissolved prior to determination of theamount of dyes associated with them. The advantages of such an assay are that: ... halfmaximal effect of NBD-Cl was observed at concentra-tions of 3–5 lm, irrespective of the rhodamine concen-trations that the cells were exposed to, indicating thatthe direct effect of the NBD-Cl...

Tài liệu Báo cáo khoa học: A facile method for expression and puriﬁcation of the Alzheimer’s disease-associated amyloid b-peptide pdf

... E22Q and D23N), and the availability of a rapid and simpleexpression and purification protocol will facilitatelarge-scale investigations of the molecular determinants of aggregation and fibrillation. ... buffer supplied with the enzyme, and contained Aba, Abb and Abcat40 nm each, and the start and stop primers Abstart and Abstop at 600 nm each, and 200 lm each of dATP, dCTP,dGTP and dTTP. The ... cut-offcentrifugal filtration device. When a solution of Ab(M1–40) of approximately 40 lm was concentratedapproximately eightfold, approximately 75% of thepeptide was recovered at a concentration...

Báo cáo khoa học: Human telomeric G-quadruplex: thermodynamic and kinetic studies of telomeric quadruplex stability potx

... equilibrium titrations with KCl and NaCl and bymulti-wavelength stopped-flow kinetics. Cation bind-ing was cooperative with Hill coefficients of 1.5–2.2in K+ and 2.4–2.9 in Na+, with half-saturation ... signalthat the denaturation process is not two-state, and likely has intermediate states that are significantly pop-ulated. Singular value decomposition provides an addi-tional test of the two-state ... transitions. Attempts to ‘correct’ thesloping baselines that arise from such complicationswould lead to oversimplification of the true reactionmechanism, and to a loss of information. Even withoutsuch...

Báo cáo khoa học: A study on genomic distribution and sequence features of human long inverted repeats reveals species-speciﬁc intronic inverted repeats pptx

... observed feature in LIR arms. Consideringthat the LIRs probably have essential functions and drive the speciation of primates, we studied the degree of conservation and species specification of the ... [33].The LIRs within genes were identified and the ratio of their relative distance to exon–intron boundaries was calcu-lated. Here, a ratio approaching to 0 indicates the relativedistance ... process and help to createcomplicated splicing patterns [39]. On the other hand,CA-tracts and CA-rich sequences are confirmed to beregulators for alternative splicing. One study showedthat the...

Báo cáo khoa học: Residues affecting the chloride regulation and substrate selectivity of the angiotensin-converting enzymes (ACE and ACE2) identiﬁed by site-directed mutagenesis pot

... 50 and 400 l m were incubated with the vari-ous forms of ACE2 and tACE. The enzyme concentrationwas adjusted to ensure that < 15% of the substrate was con-sumed at the lowest substrate ... Journal compilation ª 2008 FEBSAltered enzymatic activity of the mutant forms of tACE and ACE2 With angiotensin I as the substrate, and at 100 mmNaCl, the physiological concentration of chloride ... removal of the positive charge of this residue by the creation of an R514Q mutant pro-duces an enzyme that, compared with the wild-type,shows twofold greater activity with the substrate angio-tensin...

Báo cáo khoa học: Puriﬁcation, characterization, cDNA cloning and nucleotide sequencing of a cellulase from the yellow-spotted longicorn beetle, Psacothea hilaris ppt

... 5.9 and 7.7, respectively.Enzymatic degradation of cellulose and its derivativesbyP. hilariscellulaseTo investigate the ability of P. hilaris cellulase to degradecellulose and its derivatives, ... starting with an ATG codon at position 38 and ending with a TAA codon at position 1014. A poly(A) tail and typical polyadenylation signal were found. Two poten-tial N-glycosylation sites, N270 and ... elution and concentration of theprotein solution containing the cellulase activity after nativePAGE, the solution was mixed with sample buffer for SDS/PAGE without reducing agent and incubated at...

Báo cáo khoa học: Structural insight into the evolutionary and pharmacologic homology of glutamate carboxypeptidases II and III ppt

... movement of the ‘glutaratesensor’ upon binding of a glutamate (or glutamate-like) moiety in the S1¢ pocket. In GCPIII, the opening of the ‘glutarate sensor’ is associated with a reposition-ing of ... phosphinate group of EPE (and the sulfate group of Mops), which mimicsthe tetrahedral transition state of the reaction [31],coordinates the active site Zn2+ions with interatomicdistances of 1.9 A˚(O1 ... structural datafor the latter enzyme and its complexes with variousligands should prove immediately useful, even thoughdetailed biological and physiological data mightbecome available only later.In...

Báo cáo khoa học: Tec family kinases: Itk signaling and the development of NKT ab and cd T cells potx

... Itk and those that express atransgene for PLZF have a developmental block instage 2 of iNKT cell maturation, all of which results ina severe reduction in the number and frequency of mature and ... become mature i NKT cells. Itk and Txk are involved in the ﬁnal maturation of i NKT cells, which may be through regulating of the same pathway of TCR signal strength affecting the expression of transcription ... of iNKT cells, partic-ularly the Itk binding site Y145 [29]. Thus the absence of the Itk signaling pathway results in reduced NFATactivation and expression of NFAT-regulated genes[30]. NFATc1...

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