báo cáo khoa học: " In vitro analysis of the cytotoxicity and the antimicrobial effect of four endodontic sealers" ppt

Báo cáo khoa học: In vitro analysis of the relationship between endonuclease and maturase activities in the bi-functional doc

... statement and suggests that the RNA binding site is still available and itsbinding afﬁnity is not altered by the presence of DNA in the DNA binding site.Interestingly, when 3 nMRNA and 10 nMDNA ... I and group II introns frequently contain openreading frames (ORFs), which are either free-standingwithin the intron itself or are in- frame with the preceding 5¢exon [1,2]. While some of these ... not bind the same singlebinding site, rather that I-AniI appears to contain twobinding sites.Keywords: Aspergillus nidulans; homing endonuclease;RNA binding protein; DNA sliding; RNA splicing.Group...

báo cáo khoa học: " In vitro analysis of the cytotoxicity and the antimicrobial effect of four endodontic sealers" ppt

... decrease of the pH in the peri-apical tissues, and thus there are special demands for the sealing agent s [31]. The major task in reducing the bacterial load, concentrated in the apical region of the root ... Medicine 2011, 7:15http://www.head-face-med.com/content/7/1/15Page 3 of 9RESEARC H Open Access In vitro analysis of the cytotoxicity and the antimicrobial effect of four endodontic sealersInes ... Callaway, Benjamin Briseño and Brita Willershausen*AbstractIntroduction: The aim of this study was to investigate in vitro the cytotoxicity and antibacterial properties of four different endodontic...

Báo cáo khoa học: " In vitro analysis of expression vectors for DNA vaccination of horses: the effect of a Kozak sequence" docx

... focused by repeated intramuscu-lar and intradermal injection with the HSA gene on the G1 and H1 vectors [23]. According to this and the results of others in the field some combination of immunizations,different ... drafting the manuscript. VS: participated in the design of the study, molecular cloning, cell transfec-tions and drafting the manuscript. SI: participated in the design of the study and drafting the ... times in the cell lines and for obtaining the results shown in figure3 the vectors were transfected into all the cells at the sametime point.2.4. Western blot The expression of HSA and CAT...

Báo cáo khoa học: In vitro expansion of DNA triplet repeats with bulge binders and different DNA polymerases pdf

... triangularprism binding pocket, with its amino sugar anchored in the major groove of the DNA pointing toward the 3¢-bulge-ﬂanking base pair.We were interested in small molecules that can selec-tively bind ... overhanging nucleotides wereexcised completely from the 3¢-terminus in the anneal-ing oligomer, and consequently, no expanded bandwas observed during incubation. Doxorubicin did notinhibit the ... of an overall bending of the DNA backbone[33,35]. Both DDI-1A and DDI-1B exhibited bindingbehaviors obviously different to that of the bulgeDNA host. The addition of DDI-1A and DDI-1B tothe...

Báo cáo khoa học: In vitro embryonic developmental phosphorylation of the cellular nucleic acid binding protein by cAMP-dependent protein kinase, and its relevance for biochemical activities pdf

... hpfembryos were incubated during 15 min with different kinaseinhibitors at 30 °C and then used to analyse in vitro recom-binant CNBP phosphorylation in kinase buffer containing100 lm ATP and 5 lCi ... affects the properties of a variety of proteins with common biochemical activities, such as the binding to single-stranded nucleic acids and the remodelling of their secondary structures.A model involving ... November2006)doi:10.1111/j.1742-4658.2006.05596.x The zinc-ﬁnger cellular nucleic acid binding protein (CNBP) is a strikinglyconserved single-stranded nucleic acid binding protein essential for normalforebrain formation during mouse and...

Báo cáo khoa học: In vitro gamma-secretase cleavage of the Alzheimer’s amyloid precursor protein correlates to a subset of presenilin complexes and is inhibited by zinc potx

... [22–25]. Since a functioning zinc-bind-ing domain may be present in the Ab sequence of C99,we hypothesized that zinc may affect the oligomeriza-tion state of C101-3FLAG. Therefore, the molecularmass ... within27 Da of the calculated molecular mass. Finally the same line was used to predict the molecular mass of athird FLAG-reactive protein between alpha and c-3FLAGproteins as 11.1 kDa. In vitro ... prepared using C101-3FLAG, c-3FLAG and e-3FLAG standards with the resulting line having a correlation coefﬁcient of 0.998. Thisline was used to predict the molecular mass of the CTFmigrating faster...

Báo cáo khoa học: In silico analysis of the adenylation domains of the freestanding enzymes belonging to the eucaryotic nonribosomal peptide synthetase-like family pot

... pair of A domains and PCPs and catalyze the formation of the peptide bond between the upstream amino acyl orpeptidyl moiety tethered to the phosphopantetheinylgroup and the free amino group of the ... The structural and functional roles of the residues involved in the substrateselection and binding are proposed through the analysis of the predictedinteractions of the model active sites and ... N-terminal domains are homologousto the A and T domains from NRPS systems and the C-terminal part contains a redox cofactor bindingsite for either pyrroloquinoline quinone (PQQ) orNADPH. In particular,...

Báo cáo khoa học: In vitro and in vivo self-cleavage of Streptococcus pneumoniae signal peptidase I pot

... for the ﬁrst time that the self-cleavage of SPase I is happening within the S. pneumoniae cells in all the growth phases, and the cells maintain the active SPase I at the highest level in the ... study, we wereinterested in investigating the effect of phospholipid on the self-cleavage of S. pneumoniae SPase I. In the pres-ence of phospholipid, the self-cleavage occurred predom-inantly at ... itssubstrates. Therefore, the self-cleavage of SPase I is believedto inactivate the activity of the enzyme. In vivoself-cleavage of S. pneumoniaeSPase IAs the in vitro self-cleavage inactivates the...

Báo cáo khoa học: In vitro selection and characterization of a stable subdomain of phosphoribosylanthranilate isomerase potx

... fusion protein immediately after the arginine of the new epitope, and removed the fragment of the PRAI (ba)8-barrel corresponding to a6(ba)7)8. The remaining fragment of PRAI consisted of 130 ... importantly, the residues contributing to one of the hydrogen bonds in the core of the barrel, Lys258(b1) and Gln332 (b4), remain in trPRAI. It is temptingto speculate that, in the absence of the salt ... accommodate insertion of the FLAG epitope without signiﬁcantly perturbing the folding of the underlying (ba)8-barrel. This was inves-tigated by comparing the far-UV and near-UV CDspectra of PRAI and...

Báo cáo khoa học: In vitro characterization of a plastid terminal oxidase (PTOX) pot

... more fully, the properties of PTOX in vitro. In this report, we produced the protein in E. coli and studied its substrate preferences, the factors in uencing its activity and the nature of suitable ... probably the consequence of the increase in the overall quinone reducing activity of the membranes and not a direct effect on PTOX activity. This is corrobor-ated by the fact that the ratio of O2consumption ... This might explain theirdifferent substrate preferences. A fuller understanding of quinone–protein interactions in this class of enzyme may beachieved by testing the effect of sequence modiﬁcation...

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