báo cáo khoa học: " Production and quality of clinical practice guidelines in Argentina (1994–2004): a cross-sectional study" pps
báo cáo khoa học: " Production and quality of clinical practice guidelines in Argentina (1994–2004): a cross-sectional study" pps
... ScienceOpen AccessResearch article Production and quality of clinical practice guidelines in Argentina (1994–2004): a cross-sectional studyMar a Eugenia Esandi*1, Zulma Ortiz1, Evelina Chapman1, ... Universidad de Buenos Aires, Argentina and 4Hospital de Pediatr a "Prof. Dr. Juan P. Garrahan", Buenos Aires, Argentina Email: Mar a Eugenia Esandi* - eesandi@infovia.com.ar; Zulma Ortiz ... Klazinga N, Makela M, Zaat J, for the AGREE Col-laboration: Towards Evidence-Based Clinical Practices. Aninternational survey of 18 clinical guideline programs. Inter-national Journal for Quality...
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Tài liệu Báo cáo khoa học: Production and characterization of a secreted, C-terminally processed tyrosinase from the filamentous fungus Trichoderma reesei ppt
... GTT GGA ATT CCA TCA TCA TCATCA TCA TCA GGG CAC GAC ACA CAT CCC C; and reverse primer, GAT CGG TAC CTC ATT ACA GAGGAG GGA TAT GGG GAA C. The PCR reaction wasdone as described above. The amplified ... tyrosinase was alsoinactivated completely within 20 min [40]. In general,mammalian and plant-derived tyrosinases are not verythermostable; even a short incubation at 70–90 °C inac-tivates ... hydroxylation of aromaticamines by tyrosinase. Biochemistry 26, 8567–8571.63 Ga˛sowska B, Paweł K & Wojtasek H (2004) Interaction of mushroom tyrosinase with aromatic amines, o-dia-mines and...
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Báo cáo khoa học: Production and utilization of hydrogen peroxide associated with melanogenesis and tyrosinase-mediated oxidations of DOPA and dopamine docx
... containingDOPA and tyrosinase, the rate of substrate oxidationFig. 3. Representative chromatograms of the autoxidation and tyrosinase-mediated oxidations of DOPA, with and without cata-lase. ... of NaCl ⁄ Pi(pH 7.4) thatlacked catalase (A and B) and those with catalase (C). Arrows indi-cate times when dopamine was incorporated in the reaction mix-tures. Pulse voltage was maintained ... Production and utilization of hydrogen peroxideassociated with melanogenesis and tyrosinase-mediatedoxidations of DOPA and dopamineMaristella Mastore1, Lara Kohler2 and Anthony J. Nappi21...
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Báo cáo khoa học: Production and characterization of a thermostable L-threonine dehydrogenase from the hyperthermophilic archaeon Pyrococcus furiosus docx
... (5¢-GCGCGGGATCCTCATTTAAGCATGAAAACAACTTTGCC, antisense), containing NcoI and BamHI sites (underlined in the sequences). In order tointroduce an NcoI restriction site, an extra alanine codon(GCA) was introduced ... preculture was used toinoculate 1 L Luria–Bertani medium with kanamycin and spectinomycin (both 50 mgÆL)1) in a 2-L conical flask and incubated in a rotary shaker at 37 °C until a cell density of A 600¼ ... effect of several salts, metals and inhibitors on theinitial activity of Pf-TDH was checked using butane-2,3-diol as substrate in the standard oxidation reaction and acetoin in the reduction reaction....
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Báo cáo khoa học: Production and characterization of a noncytotoxic deletion variant of the Aspergillus fumigatus allergen Aspf1 displaying reduced IgE binding ppt
... anti-gens.DiagnosisAspf1D(7–22)vs. Aspf1 a a-sarcinvs. Aspf1 a a-sarcinD(7–22)vs. Aspf1 a a-sarcinD(7–22)vs. a- sarcin a Asthma 33 33 50 20Cystic fibrosis 50 30 89 33ABPA 20 33 70 22 a Data calculated ... (5¢-GTCGTCTTGGATCCTCTCGAGTCTCAATGAGAACACAGTCTCAAGTC-3¢). These primers contained BstEII and BamHI sites and were used to generate a fragment that wascloned in the same sequencing and expression ... a- sarcin and a- sarcin D(7–22). The inhibitor amount is plotted in logarithmic scale. The standard deviation of the measurements isshown.Variants of the A. fumigatus allergen Aspf1 L. Garcia´-Ortega...
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Báo cáo khoa học: "Production and purification of immunologically active core protein p24 from HIV-1 fused to ricin toxin B subunit in E. coli" ppt
... HIVvaccine. The availability of an optimal adjuvant and carrier to enhance antiviral responses mayaccelerate the development of a vaccine candidate against HIV. The aim of this study was toinvestigate ... Lehar SM, MacCafferty J, Chiswell D, Blattler WA, GuildB: Phage display of ricin B chain and its single bindingdomains: system for screening galactose-binding mutants.Proc Nat Acad Sci USA ... as template since lectinsdo not contain introns. The forward (5' CCG CAT GAATTC ATG GCT GAT GTT TGT ATG GAT CCT GAG CCCATA 3') and reverse primers (5' ACC TGC CTA TCA CTCGAG...
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Tài liệu Báo cáo khoa học: Models and mechanisms of O-O bond activation by cytochrome P450 A critical assessment of the potential role of multiple active intermediates in oxidative catalysis doc
... action of superoxide dismutase or catalase, implicating theinvolvement of a peroxo adduct in catalysis [243]. In fact,reactivity was enhanced when H2O2was the oxidant in p lace of reductant and ... (1983) Mechanisms of extrahepaticbioactivation of aromatic amines: the r ole of he moglobin in theN-oxidation of 4-chloraniline. In Extr ahepatic Drug Me tabolism and Chemical Carcinogenesis ... enzyme variant stillmediated N-oxygenation of the tertiary arylamine at a rateless than half that of the wild-type-catalyzed reaction [142],so that reasonable interpretation o f the data seems...
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Báo cáo khóa học: Cloning and expression of murine enzymes involved in the salvage pathway of GDP-L-fucose ppt
... skilledtechnical assistance in molecular biology, and Kati Vena¨la¨inen and Leena Penttila¨for assistance in HPLC and MALDI-TOF MS analysis.The work was supported in part by Research Grants of the Academy ... of fucokinase were subcloned intothe XbaI site of a pQM vector containing a C-terminalE2-Tag /A (Quattromed Ltd, Tarto, Estonia). The forwardprimer was 5¢-ATCTCTAGAATGGAGCAGTCAGAG-3¢ and the ... reversetranscription, was 5¢-TAGCAGCAGACTTGAAGAGGTA-3¢. PCR was performed by using the forward primer5¢-GCCAGAATGGAGCAGTCAGAGGGAGTC-3¢ and the reverse primer 5¢-GCAGCTCTAGGTGGTGCCCACTTCAGAG-3¢....
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Báo cáo khoa học: " Identification and prevalence of Ehrlichia chaffeensis infection in Haemaphysalis longicornis ticks from Korea by PCR, sequencing and phylogenetic analysis based on 16S rRNA gene" docx
... largely based on the combined evaluation of clinical signs, laboratory and epidemiological data. Since mostphysicians are unfamiliar with HME, this disease is oftenmisdiagnosed and many cases ... ticks in Japan. J Clin Microbiol 2000, 38, 1331-1338.28. Uhaa IJ, MacLean JD, Greene CR, Fishbein DB. A case of human ehrlichiosis acquired in Mali: clinical and laboratory findings. Am J Trop ... sequences of 20 representative E. chaffeensisstrains available in the GenBank database showed that EC-PGHL was 100% identical or similar to the Arkansas(AF416764), the Sapulpa (U60476) and the...
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Báo cáo khoa học: "Ecology and ecophysiology of circum-Mediterranean firs in the context of climate change" ppsx
... that A. cephalonica, A. cilicica and A. nordmanniana exhibit a range of indices, with the lowest near to 30. Meanwhile A. alba, A. bornmulleriana, A. equi-trojani, A. marocana, A. pinsapo and A. borisii ... burst: Abies cephalonica and Abiescilicica; average bud burst: Abies alba, Abies numidica,Abies marocana, and Abies pinsapo; late bud burst: Abiesnordmanniana and Abies bornmulleriana. The ... forestspecies as shown in figure 2, notably in the case of A. cephalonica, A. bornmulleriana and A. cilicica.Natural regeneration of firs is usually easy except at therange boundaries and in certain special...
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