... cofactorthat interacts with the members of the Hsp70 family.It accelerates the dissociation of the ADP–Hsp70 com-plex, and therefore an increase in Hsp40 concentrationmay cause an increase in Hsp70 ... in cell protec-tion against oxidative injuries [42], a typical feature of chilling injury [43]. In addition, sHsps are effective in preserving the integrity of the actin cytoskeleton andmicrofilaments ... threshold(Ct), estimates were obtained using the absolute quantifi-cation module in the software package. The PCR reactionswere performed in a final volume of 10 lL containing 1 lL of cDNA sample, 0.4 lm...