... F: On the Line New York: SEMIOTEXT[E]; 1983.
doi:10.1186/1744-8603-7-2
Cite this article as: Iriart et al.: The creation of the health consumer:
challenges on health sector regulation after managed ... of 12
RESEARC H Open Access
The creation of the health consumer: challenges on
health sector regulation after managed care era
Celia...
... specification of minimum training,
and other condition s for the medical profession. Such
regulations are likely to influence the ability of profes-
sionals and establishments to supply health services ... this region and also the extent to
which the European Commissi on is in a position to
negotiate on behalf of the entire Union. The non-applic-
ability of the ser...
... in the metal-
bound ApeSOD. This implies that the conformation
around the active site of ApeSOD is independent of
the presence of a metal cofactor.
Figure 3D shows the superimposition of the ... °C
(triangles) in a solution containing various concentrations of ethanol.
The residual activities after the incubation are plotted against the
ethanol concentration.
Cry...
... 6B)
disappeared after the addition of 10 mm HU (final
concentration) to R2F. Only the Mn(II) artefact was
observed after the addition of HU (Fig. 6C). This sig-
nal is similar to that of a control solution of ... is a strong promotor
in C. ammoniagenes as well. Because of high expression
from the tac promotor, the proposed function of man-
ganese in the transcriptio...
... to
catalytic regulation, but also the thermostability of
TtAK. Comparison of the crystal structures of TtAKb
and CgAKb was expected to elucidate the mechanism
of the elevated thermostability of TtAKb.
In ... AKs, only one of the eight-stranded
b-sheets is involved in effector binding. Determination
of the crystal structure of the regulatory subunit of
TtAK (Tt...
... and E97 of the second
subunit. The backbone NH group of R98 forms a
hydrogen bond with the backbone CO of F102. Fur-
thermore, the orientation of the side chain of the two
residues brings the guanidinium ... interaction (Fig. 9B) [49].
The above discussion rationalizes the observed effects
of the Y74W mutation in PfTIM on the stability of the
dimeric st...
... PH5CH8 cells. First, we
confirmed the effect of NS3-4A on the activation of
the IFN-b gene promoter by the Cardif exogenously
expressed in PH5CH8 cells. The results of the lucif-
erase reporter ... USA) were used for
detection of the phosphorylated IRF-3. The dimerization of
exogenous IRF-3 was detected by anti-EGFP monoclonal
serum (JL-8; Clontech).
Preparation of...
... because they correspond to the regions in
self-splicing introns that bind the exon substrates. The sites of
catalysis for the first step (substrate cleavage) and the second step
(exon ligation) are ... isolate the first reaction step
(substrate cleavage) by preventing the second reaction
step (exon ligation). In addition, the product of the
first reaction step is actually...
... display nicely the interaction of the S1 site, but
do not interact with the S2 site of these proteinases.
By contrast, the aspartates in positions P2 and P3 of
the chloromethylketone occupy the S2 and ... for the function of mem-
bers of this intriguing and emerging subfamily of
serine proteases.
Experimental procedures
Cloning, expression and purification
Cloning, ex...
... b8-strand of one monomer into a b-sheet of
the other monomer. The position of the zinc ion is indicated by a
circle. (B) Schematic diagram of the dimer. Each monomer is
shown with a different shade of ... On the
other hand, the low degree of conservation in the loop
regions near the active site could be associated with
differences in the substrates used by the...