... titration step, f0before addition of any ligand, and ffat the end of the titration. Fitting of the curveswas performed using the GRAPHPAD PRISMsoftware(GraphPad Software, Inc.) and a one-site ... required[30].It is of interest to investigate the structural determinants of the altered regulation of M. pneumoniae HPrK/P. With the crystallization and solution of the 3D structure of the protein ... binding of Fru1,6P2 does not affect the affinity of B. subtilis HPrK/P for ATP [16,29],an altered affinity for ATP was observed in this study for the M. pneumoniae enzyme. The presence of Fru1,6P2may...