... CBB staining; lane B,Stains-all staining; lane C, negative staining; lane D, Methyl greenstaining. Arrows on the right side of the lanes indicate the positionof the 52 kDa component. A weakly ... developed by Kitano [45,46], in which super-saturation for CaCO3could be maintained only in the ori-ginal solution by bubbling CO2gas, followed by a gradualdecline in saturation by CO2removal. ... Phosphoprotein of oyster (Crassos-trea verginica) shell organic matrix. In Hard Tissue Min-eralization and Demineralization (Suga S & Watabe N,eds), pp. 171–187. Springer-Verlag, Berlin Hederberg,Tokyo.28...