... Todistinguish total binding from non-specific binding, cells wereincubated for 10 min in either PBSCM alone or PBSCMcontaining 200 lm cocaine for inhibition of DAT or 200 lmS-citalopram for inhibition ... yielding the supernatant LS2, containing the cytosol-enriched fraction, and the pellet LP2, which isenriched in synaptic vesicles. The protein concentrationswere measured using the bicinchoninic ... which results in a deple-ting of DA in their projection area in the striatum[1,2]. The loss of dopaminergic neurons plays an essen-tial role in causing motor dysfunction, as demonstratedby...