... with the 3¢ UTR of target genes. Further-more, the 2–8 nucleotides of miRNA, known as the ‘seed region’, have been suggested to be the mostimportant region for target recognition [16]. Therefore,we ... vector. After incubation for 24, 48 and 72 h, the cells were incubated with 20 lL of MTT (at afinal concentration of 0.5 mgÆmL)1)at37°C for 4 h. The medium was removed and the precipitated formazan ... 2010 The Authors Journal compilation ª 2010 FEBSHowever, for the other two potential binding sites,miR-23a ASO or pcDNA3 ⁄ pri-23a affected the inten-sity of EGFP fluorescence in the vectors...