Báo cáo y học: "Rapid and persistent selection of the K103N mutation as a majority quasispecies in a HIV1-patient exposed to efavirenz for three weeks: a case report and review of the literature" pot
Báo cáo y học: "Rapid and persistent selection of the K103N mutation as a majority quasispecies in a HIV1-patient exposed to efavirenz for three weeks: a case report and review of the literature" pot
... persistent selection of the K103N mutation as a majority quasispecies in a HIV1-patient exposed to efavirenz for three weeks: a case report and review of the literatureEnnio Polilli1, Giustino Parruti2*, ... strain as the dominant quasispecies after very short exposure to efavirenz in vivo. Case presentation: A 55-year-old Caucasian man was switched to efavirenz, zidovudine and lamivudine in February ... K103N mutation persisted for six years as dominant quasispecies, in the absence of selective pressure, after administration of EFV for a year and a half. In such a case, the authorspostulated a...
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Báo cáo Y học: Expression and purification of the recombinant subunits of toluene/ o-xylene monooxygenase and reconstitution of the active complex potx
... formation.Enzymatic assays of Tomo F reductase activityNADH acceptor reductase activity of Tomo F was assayedspectrophometrically using Tomo C as electron acceptor.Assays were performed at ... (Pharmacia Biotech). The molecular mass markers used as standards for gelfiltration chromatography were b-amylase (200 kDa),aspartate aminotransferase (90 kDa), ribosome inactivatingprotein ... itsregulatory role in the complex. In fact, the 3.6-fold increase in the ability of Tomo H to transform p-cresol into 4-MC, in the absence of any capability of Tomo D to transferelectrons, can be attributed...
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Báo cáo Y học: Structural and serological relatedness of the O-antigens of Proteus penneri 1 and 4 from a novel Proteus serogroup O72 pptx
... polysaccharides in a yield of 20% of the LPS mass.Rabbit antisera and serological assaysPolyclonal O-antisera were obtained by immunization of rabbits with heat-inactivated bacteria of P. penneri 1and4 according ... and 2.06.Therefore, the polysaccharide has a tetrasacchariderepeating unit containing one residue each of D-Glc and D-Gal and twoD-residues of GalNAc. The 1H- and 13C NMR spectra of the ... 1Sugar analysis of the polysaccharide f rom P. penneri 1revealed glucose and galactose in almost equal amounts as well as 2-amino-2-deoxygalactose. All sugars were assigned to the Dseries using...
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Báo cáo Y học: Synthesis and turn-over of the replicative Cdc6 protein during the HeLa cell cycle potx
... left). It canassumed that p art of t he labeled c ytoplasmic hCdc6p movedinto the nucleus and bound to ch romatin, but another p artmay h ave remained in the c ytoplasm to be degraded orFig. ... Forschungs-Gemeinschaft.REFERENCES1. Neuwald, A. F., Aravind, L., Spouge, J.L. & Koonin, E.V. (1999)AAA+: a class of chaperone-like ATPases associated with the assembly, operation, and d isassembly of ... lock into G 1 and S p hase (Fig. 4A) . Wefound a gradual increase of chromatin-bound hCdc6pduring G1 phase followed b y a d ecrease in early S phase[24]. With the continuation of S phas e,...
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Báo cáo Y học: Engineering and mechanistic studies of the Arabidopsis FAE1 b-ketoacyl-CoA synthase, FAE1 KCS pot
... asparagine resulted in inactiveenzyme.Taken together, the analysis of the decarboxylationactivity and characterization of the mutants of the putativecatalytic triad strongly support the hypothesis ... the b-ketoacylsynthase domain of the multifunctional animal fatty acidsynthase [40] in which decarboxylation of malonylsubstrate is dependent on the binding of the acyl substrate to the active-site ... still capable of stabilizing the thiolate of theactivesitecysteine.Theloweractivity,comparedtothewild-type, of the histidine to glutamine mutant of chalconesynthase has been attributed to an increase...
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Báo cáo y học: "Reliability and psychometric properties of the Greek translation of the State-Trait Anxiety Inventory form Y: Preliminary data" pps
... current preliminary study was to assess the reliability and the psychometric properties of the Greektranslation of the State-Trait Anxiety Inventory (STAI) –form Y. Materials and methods The present ... and 13%, that is two thirds of explained variance. Factor 1 corresponds to a well-beingfactor, factor 3 to trait and factor 4 to state anxiety. The restfour factors are rather residual factors ... State-Trait Anxiety Inventory form Y: Preliminary dataKonstantinos N Fountoulakis*, Marina Papadopoulou, Soula Kleanthous, Anna Papadopoulou, Vasiliki Bizeli, Ioannis Nimatoudis, Apostolos Iacovides...
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Báo cáo y học: "Clinical and neurophysiological study of peroneal nerve mononeuropathy after substantial weight loss in patients suffering from major depressive and schizophrenic disorder: Suggestions on patients'''' management" pptx
... preparation of this manuscript. NK, TZ, and PKperformed the examinations on the patients. AP and GCKreviewed the laboratory records and the patients' history.AP prepared the initial and the ... fibular head seg-ment in six (cases 2–5, 9). In cases 6 and 8, the findingswere indicative of axonal damage. The amplitude of the sensory potential of the superficial peroneal nerve wasabnormally ... axonal damage can lead to pro-longed or permanent paralysis and atrophy and thus need to be evaluated for an eventual surgical intervention. The neurophysiological examination can establish the...
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Báo cáo y học: " Dimensionality and scale properties of the Edinburgh Depression Scale (EDS) in patients with type 2 diabetes mellitus: the DiaDDzoB study" pot
... discriminationpower. This assumption is unrealistic for the EDS, as shown by the varying item-factor loadings in the factoranalysis and the varying scalability coefficients in Mokkenscale analysis. ... participated in data preparation, statistical analysis, writing the manuscript, and designing the tables; WE participated in statistical analysis and writing the manuscript; GN participated in ... starting points in any IRT analysis, particularly for the purposes of (exploratory ) dimensionality analysis and early identification of malfunctioning items. For the nonparametric IRT analyses, we...
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Báo cáo y học: " Sequence and phylogenetic analysis of H7N3 avian influenza viruses isolated from poultry in Pakistan 1995-2004" pot
... with increased mortality and decreased egg production. Avian Pathol 2003, 32:285-289.7. Mase M, Imada T, Sanada Y, Etoh M, Sanada N, Tsukamoto K, Kawaoka Y, Yamaguchi S: Imported parakeets harbor ... RG: Survey of the hemagglutinin (HA) cleavage site sequence of H5 and H7 avian influenza viruses: amino acid sequence at the HA cleavage site as a marker of pathogenicity potential. Avian Dis ... the capital city of Islamabad. The disease was controlled within 4-5 months by massvaccination with a vaccine prepared from a field isolate[4]. Then in November 1998 an outbreak that was lateridentified...
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Báo cáo y học: " Biochemical and virological analysis of the 18-residue C-terminal tail of HIV-1 integrase" pot
... at the 5' end of the strand that becomes joined to Integrase proteins and in vitro integration assaysFigure 2Integrase proteins and in vitro integration assays. (A) Purified proteins (approximately ... end were used as viral DNA substrates. AE143 (5'-ACTGCTAGAGATTT-TCCACACTGACTAAAA) and AE191 (5'-TTTTAGTCAGT-GTGGAAAATCTCTAGCAG) were annealed prior to filling -in the 3' recess ... harbored three stop codons afterVif residue Asn-19, was constructed by PCR using PfuUltra DNA polymerase (Stratagene, La Jolla, CA) and primers AE1064 (5'-ACAGGATGAGGATTAACTGATGA-TAAGCTTTAGTAAAACACCATATG)/AE1065...
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