báo cáo khoa học: "Three-dimensional kinematic motion analysis of a daily activity drinking from a glass: a pilot study" pdf

Tài liệu Báo cáo khoa học: "Subjectivity and Sentiment Analysis of Modern Standard Arabic" doc

... 2011.c2011 Association for Computational LinguisticsSubjectivity and Sentiment Analysis of Modern Standard ArabicMuhammad Abdul-MageedDepartment of Linguistics &School of Library & Info. ... signiﬁcant improvement inclassiﬁcation performance.2 ApproachTo our knowledge, no SSA annotated MSA data ex-ists. Hence we decided to create our own SSA an-notated data.12.1 Data set and AnnotationCorpus: ... document domain label manu-ally associated with it.2 A detailed account of issues related to the annotation taskwill appear in a separate publication.UNIQUE: Following Wiebe et al. (2004) we ap-ply...

Báo cáo khoa học: Proteomic and biochemical analysis of 14-3-3-binding proteins during C2-ceramide-induced apoptosis pot

... using mass spectrometrydata. Electrophoresis 20, 3551–3567.88 Wakeyama H, Akiyama T, Takahashi K, Amano H,Kadono Y, Nakamura M, Oshima Y, Itabe H,Nakayama KI, Nakayama K et al. (2007) Negativefeedback ... (CABI-MER, Seville, Spain). Monoclonal antibody against a- tubulin was from Sigma. Antibody against STAT3 was from Cell Signalling (Danvers, MA, USA). Antibodiesagainst VASP, HIP-55 and Smac were kindly ... cytochrome c was from BD Pharmingen(San Jose, CA, USA). Monoclonal antibody against PARPwas from Roche Molecular Biochemicals (San Francisco,CA, USA). Rabbit antibody against cytochrome c oxidasesubunit...

Báo cáo khoa học: Semi-nested PCR analysis of unknown tags on serial analysis of gene expression potx

... length of the smear is distributed from aboutmRNAmRNANBAAAAAAA-3′NBAAAAAAA-3′NBAAAAAAAModified oligo (dT)NVTTTTTTTNBAAAAAAANVTTTTTTTNBAAAAAAANVTTTTTTTNBAAAAAAANVTTTTTTTNBAAAAAAANVTTTTTTT16161616161616165′5′5′-cap ... spermatozoa were amplified byPCR with the use of Takara Ex Taq Hot Start Version(TaKaRa), with the primary library sequences serving as thetemplate. Briefly, PLF (5¢-AAGCAGTGGTATCAACGCAGAGT-3¢) ... generate the first-strandcDNA; and the other was the 5¢-cap oligonucleotide primer(5¢-AAGCAGTGGTATCAACGCAGAGTACGCGGG-3¢),which annealed to the dC-rich cDNA tail and served as anextended template...

Báo cáo khóa học: Mutational and computational analysis of the role of conserved residues in the active site of a family 18 chitinase docx

... glycosyl-transferase elucidate catalysis in the alpha-amylase family. Nat.Struct. Biol. 6, 432–436.16. Terwisscha van Scheltinga, A. C., Armand, S., Kalk, K.H., Isogai, A. , Henrissat, B. & Dijkstra, ... circulans, whereas it may be mutated toasparagine without loss of activity in other family 18chitinases [28,30]. Preliminary results of a comparativestudy of available structures of family ... chitinases also reduced activity dramatically [26,29].The pK a calculations indicate that Glu144 has a slightlyelevated pK a in the free enzyme that, at least in part,results from the vicinity of...

Báo cáo khoa học: Structural and functional analysis of the interaction of the AAA-peroxins Pex1p and Pex6p pptx

... GAATCATTATGAAGCGGTGAGAGCTAATTTTGAAGGTGCTCGTACGCTGCAGGTCGACKU1012 TATTTACAAATTTACCTATACGCTCTGAGTTGATATTACATCGATGAATTCGAGCTCGKU1130 GCTAATAACAACTAATATCACTAACAGAAGACCATTACCCCGTACGCTGCAGGTCGACKU1131 GGAGGATTTATCAAAAGCTACTTCGAAAGCTAGGAACGAACGTACGCTGCAGGTCGACKU1132 ... CGGATCCATGGTTAAAGATATTATCGAAAGGCATTTGCKU617 CCGCATGCGGCCGCTCAAGATGGTGTAAACGCACTAAGCGKU718 CACTAGCAGAACGTACTACGGTGTGGTTTATACAAGAGGGTTCCAGCTGAAGCTTCGTACGCTKU719 GATGAAAAACCATTATGTATGTCATTAAAATAAGTAGGTAGGATAGGCCACTAGTGGATCTGKU1233 ... CTTCTCTACGCCCGTGCTCKU500 GGAGGATCCGTCGACCATGACGACGACCAAGAGKU531 CCTTCTTTGATTGTGCTGCAGAACGTTGAGGCTCTATTTGGKU532 CCAAATAGAGCCTCAACGTTCTGCAGCACAATCAAAGAAGGKU616 CGGATCCATGGTTAAAGATATTATCGAAAGGCATTTGCKU617...

Báo cáo khoa học: Modular metabolic control analysis of large responses in branched systems – application to aspartate metabolism potx

... state value), is a tenta-tive range estimated from information available forArabidopsis mutants. In a mutant of Arabidopsis inwhich inhibition of AK by Lys was abolished, a six-fold increase ... responses inbranched systems – application to aspartate metabolismFernando Ortega1and Luis Acerenza21 Computational Biology, Advanced Science and Technology Laboratory, AstraZeneca, Macclesﬁeld, ... [25].MethodsLarge parameter changes produce changes in the meta-bolic concentrations and ﬂuxes. The effect that a changein the parameter p has on the steady-state value of a variable w (metabolite...

Báo cáo khoa học: Structural and mutational analysis of TenA protein (HP1287) from the Helicobacter pylori thiamin salvage pathway – evidence of a different substrate speciﬁcity doc

... (Stratagene, La Jolla, CA,USA). The primers used were: 5¢-TATATCATTCAGGATTATTTGTATCTTTTAGAATACGCTAAGGTG-3¢ (forward, the mutagenesis codon underlined) and 5¢-TTAGCGTATTCTAAAAGATACAAATAATCCTGAATGATATAAAAAC-3¢ ... H, Yao M, Watanabe N & Tanaka I (2004) Struc-ture analysis of PH1161 protein, a transcriptional acti-vator TenA homologue from the hyperthermophilicarchaeon Pyrococcus horikoshii. Acta Crystallogr ... enzyme assay for transglutaminase activity. Anal Biochem 274, 141–144.25 Gasteiger E, Hoogland C, Gattiker A, Duvaud S,Wilkins MR, Appel RD & Bairoch A (2005) ProteinIdentiﬁcation and Analysis...

Báo cáo khoa học: Antibody-based proteomics Analysis of signaling networks using reverse protein arrays pdf

... rela-tively easy to translate antibody validation by westernblotting into an array format.Although RPAs have gained popularity as an alter-native to classical western blots because of a dramati-cally ... 2Pancreas 3Pancreas 4Pancreas 5Pancreas 6Pancreas 7Pancreas 8TSC-205001000150020002500Heart 1Heart 2Heart 3Heart 4Heart 5Heart 6Heart 7Heart 8Pancreas 1Pancreas 2Pancreas ... 3Pancreas 4Pancreas 5Pancreas 6Pancreas 7Pancreas 8GSK-3α0500100015002000250030003500Heart 1Heart 2Heart 3Heart 4Heart 5Heart 6Heart 7Heart 8Pancreas 1Pancreas 2Pancreas...

Báo cáo khóa học: Mutational and structural analysis of cobalt-containing nitrile hydratase on substrate and metal binding pdf

... 431Mutational and structural analysis of cobalt-containing nitrilehydratase on substrate and metal bindingAkimasa Miyanaga1, Shinya Fushinobu1, Kiyoshi Ito2, Hirofumi Shoun1and Takayoshi ... theNational Project on Protein Structural and Functional Analysis. References1. Yamada, H. & Kobayashi, M. (1996) Nitrile hydratase andits application to industrial production of acrylamide. ... 5¢-GATCCAGTGCCAGTAGAACGGCGACTCGAG-3¢ (68r), 5¢-CACGTCGTCGTGTGCTCGCTCTGCTCCTGC-3¢ (109f), 5¢-GCAGGAGCAGAGCGAGCACACGACGACGTG-3¢ (109r), 5¢-CTCTGCTCCTGCACCCCATGGCCGGTGCTG-3¢ (114f), and5¢-CAGCACCGGCCATGGGGTGCAGGAGCAGAG-3¢(114r)....

Báo cáo khoa học: Conformational and functional analysis of the lipid binding protein Ag-NPA-1 from the parasitic nematode Ascaridia galli potx

... Timanova A, Marti T, Walter RD & Bankov I (1997)Isolation and partial characterization of a fatty-acid-Conformation, ligand binding and distribution of nematode protein Ag-NPA-1 R. Jordanova ... than for fatty acids. They nota-bly differ in their amino acid sequence from NPAs[11].Ag-NPA-1 from the parasitic nematode Ascaridagalli is a member of the NPAs family [12]. Its fattyacid ... moments of the chromophores are avail-able, a random orientation of the donor–acceptor pair wasassumed (K20.667 [30]). A value of 1.36 was taken for therefractive index n [31].MALDI-TOF analysis MALDI-TOF...

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