báo cáo khoa học: " Functional genomics and rheumatoid arthritis: where have we been and where should we go?" pdf

... rheumatoid arthritis.© 2010 BioMed Central Ltd Functional genomics and rheumatoid arthritis: where have we been and where should we go?James N Jarvis1* and Mark Barton Frank2R E VIE W*Correspondence: ... us, any survey of where we have gone and where we might go in the use of systems biology and functional genomics to understand RA must be informed by the rich and exciting wellspring of data ... Jarvis JN, Frank MB: Functional genomics and rheumatoid arthritis: where have we been and where should we go? Genome Medicine 2010, 2:44.Jarvis and Frank Genome Medicine 2010, 2:44 http://genomemedicine.com/content/2/7/44Page...

Báo cáo khoa học: Functional genomics by NMR spectroscopy Phenylacetate catabolism in Escherichia coli docx

... mutantsGenes paaF, paaH, paaI, paaJ and paaZ were targeted inE. coli K12 as previously described [11]. All gene mutationswere veriﬁed by PCR and sequencing (Microbiology and Molecular Genetics Core ... 0.3 mM) were added to 15 mL of cellsuspension. The mixtures were incubated at 30 °C undershaking (180 r.p.m.). Samples were retrieved at intervals and centrifuged. The supernatants were analyzed ... phenylacetate,phenylacetyl-CoA and 2-hydroxyphenylacetate were 20, 21 and 12 min, respectively.NMR spectroscopyThe lyophilized samples were dissolved in 0.5 mL ofa 1 : 1 (v/v) mixture of D2O and methanol-d4.1D13C-NMR...

Báo cáo khoa học: Functional reconstitution of mammalian ‘chloride intracellular channels’ CLIC1, CLIC4 and CLIC5 reveals differential regulation by cytoskeletal actin docx

... reconstitutingrecombinant human CLIC1, CLIC4 and CLIC5 in pla-nar lipid bilayers. CLIC1 and CLIC4 have previously been characterized at the single-channel level, and, inthis study, we conﬁrmed for the ﬁrst time ... channelsthat were almost equally permeable to Na+,K+ and Cl–, suggesting thatthe ‘CLIC’ nomenclature may need to be revised. CLIC1 and CLIC5, butnot CLIC4, were strongly and reversibly ... volume V0 and the total columnvolume Vtwere 4.5 and 70 mL, respectively, measured usingbromophenol blue and blue dextran. The inset Coomassie-stainedSDS ⁄ PAGE analysis shows a major band at...

Tài liệu Báo cáo khoa học: Molecular aspects of rheumatoid arthritis: chemokines in the joints of patients pdf

... SDF-1 ⁄ CXCL12 and fractalkine ⁄CX3CL1, are also known to have angiogenic proper-ties [44,58,59]. Recently, associations between thechemokine gene polymorphisms and RA have been investigated. ... isoform of CCL3 encoded bydifferent genes and a potent ligand for CCR1 and CCR5, influenced susceptibility to RA, and geneticinteraction between CCL3L1 dose and CCR5D32 wasalso found [62]. These ... inflammation, cell proliferation and angiogenesis. To date, several animal studies and human studies have shown the biological efficacy ofspecific antagonism of ligands and receptors in RA[45,63]....

Tài liệu Báo cáo khoa học: Molecular aspects of rheumatoid arthritis: role of environmental factors pdf

... synoviocytes and the development ofcollagen-induced arthritis [53].Weather conditionsAlthough no good biologic hypotheses have been proposed that might explain the association betweenRA and weather ... genetic and environmental factors. Genetic inﬂuences thatcontribute to RA susceptibility have been demonstrated both in studies oftwins and families, as well as in genome-wide linkage scans, and ... by both genetic and environmental fac-tors. Genetic inﬂuences that contribute to RA suscepti-bility have been demonstrated in both studies of twins[1] and families [2], as well as in genome-wide...

Tài liệu Báo cáo khoa học: Molecular aspects of rheumatoid arthritis: role of transcription factors ppt

... were stimulated with 10 ng ÆmL)1ofIL-1b. Interleukin-6, IL-8 and GM-CSF productionfrom FLS were suppressed in a dose-dependent man-ner in the presence of PPAR-c ligands and a PPAR-aligand, ... hyperlipidemia and hypertension, there aresome drugs that have the potential to inhibit NF-jBactivation. To seek other candidate compounds for usein an anti-RA strategy, we studied several drugs that have ... kidney and cells of the arterialwall and it is activated by ﬁbrate, fatty acids and eico-sanoids. Peroxisome proliferator-activated receptor-aligands inhibit IL-1-induced production of IL-6 and prostaglandin...

Báo cáo y học: "Functional genomics analysis of low concentration of ethanol in human hepatocellular carcinoma (HepG2) cells. Role of genes involved in transcriptional and translational processes"

... (AP1, Elk1, Stat1, SRF and NFκB) and expression of apoptotic genes (Fas receptor, Fas ligand, FADD and caspase 8) [19]. In addition, as confirmed by the functional genomics analysis presented ... STAU2, and HMGN3) genes and one down-regulated (ANK3) gene. All these genes exert their function on transcriptional and translational processes and until now none of these genes have been associated ... and NFκB) and expression of apoptotic genes (Fas receptor, Fas ligand, FADD and caspase 8) [19]. However, the effect of low concentration of ethanol on genes involved in transcriptional and...

Tài liệu Báo cáo khoa học: Functional and structural analyses of N-acylsulfonamidelinked dinucleoside inhibitors of RNase A ppt

... and biochemicals were of reagent grade or better, and were used without further puriﬁcation.Compounds 1–3 [49,50] and 4–7 [51] were synthesized asdescribed previously, and were generous gifts ... they were found to behighly soluble, and resistant to both enzyme-catalyzed and nonenzymatic hydrolysis [28,29]. Unlike this previ-ous study, however, we chose to examine sulfonamidesthat were ... RhRiIi(h), where Ii(h) and I(h) are the ith and the meanmeasurements of the intensity of reﬂection h, respectively. Rcryst= Rh|Fo) Fc| ⁄ RhFo, where Fo and Fcare the observed and calculatedstructure...

Tài liệu Báo cáo khoa học: Functional studies of active-site mutants from Drosophila melanogaster deoxyribonucleoside kinase Investigations of the putative catalytic glutamate–arginine pair and of residues responsible for substrate speciﬁcity docx

... purines and negative cooperativity towardsdThd and dCyd was observed. The Q81N mutation showed a 200- and 100-fold increase in Km, whereas kcatwas decreased five- and twofold fordThd and dCyd, ... 210–230. The loop connecting a9 and b5 (residues 195–200) was flexible and poor densitywas observed. The electron density for the mutant resi-due and the ligand was well defined. Structural super-positioning ... use.Non-radioactive nucleosides were from Sigma. Materials forcloning, PCR, DNA sequencing, assay and crystallizationwere standard commercially available products.Site-directed mutagenesis and expressionplasmidExpression...

Tài liệu Báo cáo khoa học: Functional hierarchy of plasminogen kringles 1 and 4 in ﬁbrinolysis and plasmin-induced cell detachment and apoptosis docx

... 6, Glu-Pg- and mAb 34D3-trea-ted cells. Bands in lanes 1 and 2 correspond to forms I and II of plasmin(ogen). High molecular weight bands in lanes 5 and 6 correspond tomAb A10.2- and mAb 34D3-plasminogen ... conformations and LBS accessibility toits functional properties such as binding to fibrin and cells, activation on these surfaces and the effect ofplasmin proteolytic activity on fibrinolysis and extra-cellular ... evaluate their respective contribution to Glu- and Lys-Pg binding and activation onto fibrin and cells, as well as their effects on fibrinolysis and on plas-min-induced cell apoptosis. The LBS...

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