báo cáo khoa học: "Structure and expression of the maize (Zea mays L ) SUN-domain protein gene family: evidence for the existence of two divergent classes of SUN proteins in plants" pot

báo cáo khoa học: "Structure and expression of the maize (Zea mays L.) SUN-domain protein gene family: evidence for the existence of two divergent classes of SUN proteins in plants" pot

... Murphy et al.: Structure and expression of the maize (Zea mays L. ) SUN- domain protein gene family: evidence for the existence of two divergent classes of SUN proteins in plants. BMC PlantBiology 2010 ... 10:269http://www.biomedcentral.com/1471-2229/10/269Page 18 of 22Structure and expression of the maize (Zea mays L. ) SUN- domain protein gene family: evidence for the existence of two divergent classes of SUN proteins in ... divergence of these two classes. These data alsosuggest that the PM3 proteins originated early in the life of the plant kingdom, predating the origin of floweringplants. The second, four orthologous...

Báo cáo khoa học: Structure, mRNA expression and linkage mapping of the brain-type fatty acid-binding protein gene (fabp7 ) from zebraﬁsh (Danio rerio) potx

... (200 2) Cellular retinol-binding protein type II724 R Z. Liu et al.(Eur. J. Biochem. 27 0) Ó FEBS 2003B-FABP gene (q22-q2 3) and MACS (q22. 2) are alsoclosely linked (Table 2). Some of the other genes ... Cordoba, O .L. , Santome´, J.A. & Dell’Angelica,E.C. (199 5) Molecular evolution of the multigene family of intracellular lipid-binding proteins. Biochem. Mol. Biol. Int. 36,1117–1125.Table 2. ... acid-binding proteins (FABPs), a group of lowmolecular mass (14–16 kDa) proteins encoded by a multi- gene family (reviewed in [6–8 ]). Brain-type fatty acid-binding protein (B-FABP) was ﬁrst isolated...

Tài liệu Báo cáo khoa học: Structure and function of active chromatin and DNase I hypersensitive sites pdf

... This involved progressivelyfractionating chromatin into (i) highly soluble smallchromatin fragments readily released from nuclei dur-ing digestion (fraction S 1), (ii) the bulk of the remain-ing ... (200 9) A unifying model for the selective regulation of inducible transcription byCpG islands and nucleosome remodeling. Cell 138,114–128.205 Allen MD, Grummitt CG, Hilcenko C, Min SY,Tonkin LM, ... manipulation. The ﬁrst LCR to be formally identiﬁed was the mammalian b-globin LCR, which exists as a cluster of DHSs, including a classical enhancer, several kilobasesupstream of the b-globin gene cluster...

Tài liệu Báo cáo khoa học: Structure and function of plant aspartic proteinases pptx

... beinvolved in intracellular protein turnover to increase aminoacid pools for the synthesis of speciﬁc defense-related proteins and/ or in the defense against pathogens byhydrolyzing proteins ... suggesting the involvement of theseAPs in developmentally regulated PCD. Nucellin gene expression was shown to be synchronized with degeneration of barley nucellar cells by PCD after pollination, and ... proposed that the AP could be involved in the hydrolysis of c-globulin during the initial stage of germination becauseboth proteins are similarly distributed in the seeds [50]. Asimilar role was suggested...

Tài liệu Báo cáo khoa học: Structure and membrane interaction of the internal fusion peptide of avian sarcoma leukosis virus pdf

... bilayer, including in particular the structural in uence of proline. Consistentwith other c lass I viral fus ion proteins, the IFP o f ASLVinserts into the membrane primarily as a h elix in ... PS 3) operated in the manual mode. The N-terminal free peptideswere labelled with TAMRA following the standard Fmoc-amino a cid coupling protocol with a coupling t ime of 10 h.Labelling with NBD ... aque-ous to micellar medium; more speciﬁcally, NMR resultsindicate that the helical structure is in duced mainly in the region N-terminal to proline and there is a slight kink in the helix at Pro13....

Báo cáo khoa học: Structure and function of KH domains docx

... RM & JamesTL (200 7) X-ray crystallographic and NMR studies of protein protein and protein nucleic acid interactionsinvolving the KH domains from human poly(C)-binding protein- 2. RNA 13, 1043–1051.34 ... backbone of the nucleic acid.Poly(C)-binding proteins Poly(C)-binding proteins (PCBPs) contain three type IKH domains, which appear to function independently,because they are separated by long linkers: ... For example, the Kdvalues of the KH domain of the SF1–DNA complex and the hnRNP K KH3 domain–DNA complex are 3 lm and 1 lm, respectively [22,23]. The clustering of KHdomains increases nucleic...

Báo cáo khoa học: Structure and mechanism of the ThDP-dependent benzaldehyde lyase from Pseudomonas ﬂuorescens potx

... PAGE,pooled and adjusted to a concentration of 20 mgÆmL )1 . The typical yield was 8 mg protein g )1 cell pellet. SeMet-labelled BAL was obtained by introducing the expression vector into the methionine-auxotrophic ... the gene of the benzaldehyde lyase and for helpful discus-sions, the teams of the Swiss Light Source (Villigen ⁄ CH) and of the EMBL outstation Hamburg for their helpwith data collection and ... BAL (Figs 6 and 7) as well as in the crystal structure of BFD [12]. We suggest that thisapplies for all acyloin cleavage reactions of BAL.During acyloin C–C-bond formation, on the otherhand,...

Báo cáo khoa học: Cloning and expression of the ﬁrst nonmammalian interleukin-11 gene in rainbow trout Oncorhynchus mykiss pdf

... synovial ﬁbroblasts, lung epithelial celllines and endometrial epithelial and stromal cells.TGF-b has been shown to stimulate IL-11 production in a number of cell types, including lung epithelial ... in these studies; the mononuclear cell line RTS-11 [33], the gonad cell line RTG[34], the liver cell line RTL [35] and CL-6 (a generous giftfrom Dr Benmansour, INRA, France). All trout cells ... weregrown in L- 15 medium supplemented with 100 unitsÆ mL )1 penicillin and 100 lgÆmL )1 streptomycin, and 30% fetal calfserum for RTS-11, and 10% fetal bovine serum for all the other cell lines.Bacterial...

Báo cáo khoa học: Structure and function of N-acetylglucosamine kinase Identiﬁcation of two active site cysteines pptx

... kDa protein part. The analysis of wild-type and mutated GlcNAc kinases in BL21 cytosols thereforedetected 42 kDa polypeptides in all cases (Fig. 2). The expression of functionally intact proteins ... on the sequence align-ments of glucokinase and GlcNAc kinase and the commonATP-binding domain, the GlcNAc kinase was ﬁtted into the glucokinase model using RasMol software. Figure 5 shows the ... found that the subcellularlocalization of GlcNAc kinase in ﬁbroblasts is differentfrom the localization of the enzymes of the de novopathway [7], indicating a spatial separation of the two pathways,...

Báo cáo khoa học: Structure and topology of the transmembrane domain 4 of the divalent metal transporter in membrane-mimetic environments docx

... palmitol(doxyl) stearoyl-phosphatidyl-choline (doxy-lPtdCho) lipids containing the nitroxide label on C12(12-doxylPtdCho) and paramagnetic metal ions (Mn2+ and Gd3+ ), on the intensities of NMR ... MA, USA). Palmitol(doxyl)-stearoyl-phosphatidylcholine (doxylPtdCho) lipids containing the nitroxide spin label on C12 were purchased from AvantiPolar Lipids (Alabaster, AL, USA).Circular dichroism ... rapidly and almost completelydisappeared within 2 h (except Gly6, which disappeared 6 hlater). However, the residues involved in the formation of the helix comprising of Val8–Leu21 retained their...

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