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báo cáo khoa học: " Whole-Organ analysis of calcium behaviour in the developing pistil of olive (Olea europaea L ) as a tool for the determination of key events in sexual plant " ppsx

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báo cáo khoa học: " Whole-Organ analysis of calcium behaviour in the developing pistil of olive (Olea europaea L.) as a tool for the determination of key events in sexual plant " ppsx

... al .: Whole-Organ analysis of calcium behaviour in the developing pistil of olive (Olea europaea L. ) as a tool for the determination of key events in sexual plant reproduction. BMC Plant Biology ... RESEARC H ARTIC L E Open Access Whole-Organ analysis of calcium behaviour in the developing pistil of olive (Olea europaea L. ) as a tool for the determination of key events in sexual plant reproductionKrzysztof ... molecules in plants and animals [5] and is involved in multiple signaltransduction pathways, which are fundamental for manyintercellular and intracellular interactions [6,7]. Calcium plays an essential...
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Tài liệu Báo cáo khoa học: Proteomic analysis of dopamine and a-synuclein interplay in a cellular model of Parkinson’s disease pathogenesis docx

Tài liệu Báo cáo khoa học: Proteomic analysis of dopamine and a-synuclein interplay in a cellular model of Parkinson’s disease pathogenesis docx

... analysis of dopamine and a- synuclein interplay in a cellular model of Parkinson’s disease pathogenesisTiziana Alberio1, Alessandra Maria Bossi2, Alberto Milli2, Elisa Parma1, Marzia Bruna Gariboldi1,Giovanna ... b-gal cells in the presence of catalase only (cat) or in the presence of catalase and0.250 mm dopamine for 24 h (DA). Dopamine treat-ment significantly increased the expression of a- synuc-lein ... plates)were transfected with pNF-jB-Luc plasmid (Stratagene,Santa Clara, CA, USA) (150 ngÆ well )1 ) and phRL-CMV,containing Renilla luciferase cDNA (5 ngÆwell )1 ), usingLipofectamine and OptiMEM...
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Tài liệu Báo cáo khoa học: Kinetic analysis of effector modulation of butyrylcholinesterase-catalysed hydrolysis of acetanilides and homologous esters pdf

Tài liệu Báo cáo khoa học: Kinetic analysis of effector modulation of butyrylcholinesterase-catalysed hydrolysis of acetanilides and homologous esters pdf

... acetylcholinesterase from eel and basalganglia: effect on the acetylcholinesterase and arylacylamidase activities. Biochemistry 23, 4088–4093.31 Boopathy R & Balasubramanian AS (198 5) Chemicalmodification ... potential detoxification role of the AAAactivity of BuChE needs to be addressed.Known AAAs are serine hydrolases that catalyse the deacylation of N-acyl arylamines [20,21]. SeveralAAAs have been ... hydrolysis of o-NAC is acylation [35], it follows that the activatingeffects of tyramine take place at the level of acylation. For the hydrolysis of o-NPA, both acylation and deac-ylation are partly...
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Tài liệu Báo cáo khoa học: Quantitative analysis of the experimental O–J–I–P chlorophyll fluorescence induction kinetics Apparent activation energy and origin of each kinetic step Steve Boisvert, David Joly and Robert Carpentier doc

Tài liệu Báo cáo khoa học: Quantitative analysis of the experimental O–J–I–P chlorophyll fluorescence induction kinetics Apparent activation energy and origin of each kinetic step Steve Boisvert, David Joly and Robert Carpentier doc

... numerical dataalso demonstrated that this decrease was compensated for by an increase in the J–I phase. We also observedthat half-times at 15 °C were always higher than at25 °C for all steps in all ... limitedinformation. In particular, the characteristics of the J–I phase are almost impossible to determine from vis-ual analysis of the traces. It was shown that the threephases can be quantitatively ... proceduresThylakoid membrane preparationThylakoid membranes were isolated from fresh marketspinach (Spinacia oleracea) as described by Joly et al. [9].Chl concentration was calculated following the...
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Tài liệu Báo cáo khóa học: Quantitative analysis, using MALDI-TOF mass spectrometry, of the N-terminal hydrolysis and cyclization reactions of the activation 2 process of onconase pdf

Tài liệu Báo cáo khóa học: Quantitative analysis, using MALDI-TOF mass spectrometry, of the N-terminal hydrolysis and cyclization reactions of the activation 2 process of onconase pdf

... conditions of the assay, the intramolecular cyclization of glutaminyl to pyroglutamylat the N-terminus of ONC (M2 3L) is not accelerated bylowering the pH, as observed for free L- Gln in aqueoussolutions ... can be completed in as little as 3 h at 70 °C (4 h after the addition of the exopeptidase).Preparative activationActivation of (Pyr)-ONC (M2 3L) was performed as described in the Materials and ... with an equal volume of saturated sinapinic acid in the same solvent of the matrix, and 0.5 lL of the mixturewas immediately applied to a sample probe spot.Cyclization reaction as a function of...
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Tài liệu Báo cáo khoa học: Thermodynamic analysis of porphyrin binding to Momordica charantia (bitter gourd) lectin pptx

Tài liệu Báo cáo khoa học: Thermodynamic analysis of porphyrin binding to Momordica charantia (bitter gourd) lectin pptx

... water-solubleporphyrins with lectins. In the initial studies, we character-ized the interaction of several f ree-base and metalloporphy-rins with plant lectins s uch as concanavalin A ( ConA), pealectin, ... 105M )1 .Interestingly, the fact that auxins and cytokinins function as plant growth regulators [46] suggests that these moleculesmay a ct as endogenous ligands for plant lectins. The a bility of ... pealectin, jack fruit (Artocarpus integrifolia) agglutinin (jacalin),snake gourd (Trichosanthes anguina) seed lectin (SGSL) andTrichosanthes cucumerina seed lectin (TCSL) [15–18].Momordica...
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Tài liệu Báo cáo khoa học: Subproteomics analysis of Ca2+-binding proteins demonstrates decreased calsequestrin expression in dystrophic mouse skeletal muscle pdf

Tài liệu Báo cáo khoa học: Subproteomics analysis of Ca2+-binding proteins demonstrates decreased calsequestrin expression in dystrophic mouse skeletal muscle pdf

... ÔStains-AllÕ labelling did not result in suitable mass spe ctra for the proper identification of Ca2+-binding proteins (data notshown). The analysis of ÔStains-AllÕ labelled spot no. 8, using a ... gel in a stainless steel tray. The tray was then placed on top of a hot plate and the temperature maintained at 9 0 °C for 5 min to aid the staining of protein spots. The tray was then placed ... (B) skeletal muscle labelled w ith the cationic c arboc yanine dyeÔStains-AllÕ. A comparison between the selective d ye labelling o f p ro-tein spo ts in panel (A) and panel ( B) showed that...
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Tài liệu Báo cáo khoa học: Functional analysis of the basic helix-loop-helix transcription factor DEC1 in circadian regulation ppt

Tài liệu Báo cáo khoa học: Functional analysis of the basic helix-loop-helix transcription factor DEC1 in circadian regulation ppt

... and5¢-GCAGCAGGATCCTCTAGAGAGTTTAGTCTTTG-3¢ for FLAG-DEC1; and 5 ¢-GAATTCGGCGGACCAGAGAATGGACATTTCCTCAACCATC-3¢ and5¢-TCTAGACTACAGCGGCCATGGCAAGTCACTAAAGTC-3¢ for FLAG-BMAL 1) were used for amplificationby ... t-test).TheregionsofBMAL1expressedasaGAL4fusionprotein are schematically shown in the upper panel. The basic helix-loop-helix (bHLH), PAS -A and PAS-B domains [32] are i ndicated.Interactions ... anti-FLAGimmunoglobulin (left-hand panel). The transcriptional activities of FLAG-BMAL1 and FLAG-DEC1 were confirmed by using the luciferase assay(right-hand panel). The values represent relative luciferase...
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Tài liệu Báo cáo khoa học: Control analysis as a tool to understand the formation of the las operon in Lactococcus lactis doc

Tài liệu Báo cáo khoa học: Control analysis as a tool to understand the formation of the las operon in Lactococcus lactis doc

... PCR fragment was generated using primer CP-pyk(5¢-ACGACTAGTGGATCCATNNNNNAGTTTATTCTTGACANNNNNNNNNNNNNNTGRTATAATNNNNAAGTAATAAAATATTCGGAGGAATTTTGAAATGAATAAACGTGTAAAAATCG-3 )( A, T, G, C) and ... Denmark).DNA techniquesAll manipulations were performed as described bySambrook et al. [24]. Taq DNA polymerase (New EnglandBiolabs, Frankfurt am Main, Germany) was applied for analytical purposes ... pyk andldh, coding for phosphofructokinase (PFK),pyruvate kinase (PK) and lactate dehydro-genase (LDH), respectively.B. Koebmann et al. Control analysis of the las operonFEBS Journal 272...
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Tài liệu Báo cáo khoa học: Mutational analysis of plasminogen activator inhibitor-1 Interactions of a-helix F and its neighbouring structural elements regulates the activity and the rate of latency transition pdf

Tài liệu Báo cáo khoa học: Mutational analysis of plasminogen activator inhibitor-1 Interactions of a-helix F and its neighbouring structural elements regulates the activity and the rate of latency transition pdf

... the fraction of the total amount of PAI-1 forming a stable complex with uPA, was calculated from the amount of PAI-1 required to inhibit half the uPA. The half-life of PAI-1 was finally calculated ... activity below 20 %) was added to each wellfollowed by incubation for at least 5 min to allow complexformation between uPA and PAI-1. The remaining uPAactivity in each well was determined as ... urokinase-type and tissue-type plasmino-gen activator (uPA and tPA, respectively) and as such is animportant regulator of physiological events in whichplasmin-catalysed extracellular proteolysis...
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