báo cáo khoa học: " Antimicrobial activity of spherical silver nanoparticles prepared using a biocompatible macromolecular capping agent: evidence for induction of a greatly prolonged bacterial lag phase" potx

... biocompatible macromolecular capping agent: evidence for induction of a greatly prolonged bacterial lag phase. Journal of Nanobiotechnology 2010 8:34.Submit your next manuscript to BioMed Centraland take ... than S. aureus to thekeratin-based Ag Nps.ConclusionsIn this work we have evaluated the antimicrobial prop-erties of a biocompatible macromolecular capping agent-based (keratin) Ag Np using ... a biocompatible macromolecular capping agent: evidence for induction of a greatly prolonged bacterial lag phasePeter Irwin1*, Justin Martin1,2, Ly-Huong Nguyen1, Yiping He1, Andrew Gehring1,...

Báo cáo khoa học: Antimicrobial activity of histones from hemocytes of the Paciﬁc white shrimp ppt

... vertebrates r anging from fish to humans. Histone antimicrobial activity was first demonstrated in 1958 for histones A and B purified from calf thymus, which exhibitedantibacterial activity against various ... hyaline, semigranular, and granular cells.The hyaline cells are associated with coagulation a ndphagocytosis while the semigranular and granular cells areinvolved in phagocytosis, release of ... (http://www.ncbi.nlm.nih.gov/blast) with the non-redundant database.Histone H 2A N-terminal cDNA cloningTwo degenerate forward primers (F1: 5¢-AACMGKGCMGGACTCCAG-3¢;F2:5¢-TMCGYAARGGMAACTATG-3¢) and three degenerate...

Tài liệu Báo cáo khoa học: Autolytic activity of human calpain 7 is enhanced by ESCRT-III-related protein IST1 through MIT–MIM interaction pptx

... substrates have been identified.Although calpain 7 contains a C2-like domain, it lacks a penta-EF-hand domain and is classified as an atypicalcalpain. As one of the significant structural features,mammalian ... Germany).Construction of plasmidsCloning of human calpain 7 cDNA and construction of mammalian expression plasmids for various mGFP-fusedcalpain 7 mutants (FLAG–CHMP1B, FLAG–CHMP4b,FLAG–CHMP6 ... nucleotidesequencing. For bacterial expression of GST–IST1MIM, a cDNA fragment encoding amino acids 300–364 was ampli-fied by PCR, with a pair of primers (forward, 5¢-TAGGATCCCCTGGACCCAAGCCAGAAG-3¢; reverse,...

Tài liệu Báo cáo khoa học: Antimicrobial effects of H4-(86–100), histogranin and related compounds – possible involvement of DNA gyrase ppt

... 253–257.34 Takeshima K, Chikushi A, Lee KK, Yonehara S &Matsuzaki K (2003) Translocation of analogues of the antimicrobial peptides magainin and buforinacross human cell membranes. J Biol ... buforins and pos-sibly HN. The inﬂammation is also accompanied bythe release of large amounts of ATP from stimulatedcells [13,25]. A large body of evidence indicates thatcationic antimicrobial ... characterization of histogranin, a natural peptide with N-methyl-D-aspartate antagonist activity. Eur J Pharmacol MolPharmacol 245, 247–256.15 Lemaire S, Rogers C, Dumont M, Shukla VK, LapierreC,...

Tài liệu Báo cáo khoa học: ATPase activity of magnesium chelatase subunit I is required to maintain subunit D in vivo ppt

... 4B).Presence of Xantha-gmRNA inxantha-hmutants A possible explanation for the absence of 70 kDa XAN-Gprotein in the xantha-h mutants could be that the XAN-Hprotein affects the level of Xantha-g mRNA. ... primersEXgLp67 (5¢-CGTAGATACAAACTTGTTCTCGGTAT-3¢) and EXgUp70 (5¢-GCATTTATTCCCTTCCGTGGAGACT-3¢) are separated by two introns in the chromo-somal Xantha-g DNA. Therefore a DNA fragment ampli-ﬁed from ... for Environment, Agricultural Sciences and Spatial Planning, and theMagn. Bergvall Foundation to M. H., and the Australian ResearchCouncil (grant no. A0 9905713) and an IREX award (grant no.X00001636)...

Tài liệu Báo cáo khoa học: High activity of human butyrylcholinesterase at low pH in the presence of excess butyrylthiocholine pptx

... expression for a single pK a (see the legend to Table 1 for details). The values for kcatand bkcat,ateachpH,weretakenfromﬁtting of kappvs. BTC concentration data for each mutant (data notshown).318 ... kcat activity was effectivelyzero, and bkcat activity was approaching zero (Fig. 3). Thetitration of kcatwas monophasic, with a pK a of 6.7 ± 0.09.However, the titration of bkcatwas ... clearly approach a nonzero limiting activity at low pH. Limiting activity is theplateau value in a titration curve. All of the titrations extendover a range of at least 3 pH units, indicating that...

Báo cáo khóa học: Chaperone activity of cytosolic small heat shock proteins from wheat pptx

... TaHsp17.8C-IIcontains either fewer subunits than TaHsp16.9C-I, or has a more nonglobular shape. Finite element analysis of thedata [28] estimates a molecular mass of 201 kDa for TaHsp16.9C-I and 173 kDa for ... University of Arizona ExperimentStation Funds, and American Cancer Society Faculty Research Award#FRA-420 to E. V. G. J. L. was a recipient of a National Institutes of Health Postdoctoral Fellowship. ... for TaHsp17.8C-II. These dataare consistent with a more extended shape for TaHsp16.9C-Ithan for TaHsp17.8C-II and with a dodecameric organiza-tion for TaHsp16.9C-I and an oligomer of TaHsp17.8C-IIcontaining...

Báo cáo khóa học: Antimicrobial activities of heparin-binding peptides pdf

... multiples of AKKARA or ARKKAAKA [15,20] were synthesized andtested in bactericidal assays using E. faecalis as the testorganism, and the results demonstrate that these peptides arealso antibacterial. ... visualized as a clearzone around each well after 18–24 h of incubation at 37 °C for bacteria and 28 °CforC. albicans.ForE. coli the dose–response characteristics of the RDA was used and the linearrelationship ... Yoshida, I., Tashiro, K., Monji, A. , Nagata, I., Hayashi, Y.,Mitsuyama, Y. & Tashiro, N. (1999) Identiﬁcation of a heparinbinding site and the biological activities of the laminin alpha1chain...

Báo cáo khoa học: Cytotoxic activity of nucleoside diphosphate kinase secreted from Mycobacterium tuberculosis pptx

... 477–487.15. Chopade, B .A. , Shankar, S., Sundin, G.W., Mukhopadhyay, S. &Chakrabarty, A. M. (1997) Characterization of membrane–asso-ciated Pseudomonas aeruginosa Ras-Like proteins Pra, a GTPbinding ... CAC GGATCC GTG ACC GAA-3¢, carrying BamHI at its 5¢-end and a reverse primer 5¢-TCC GGA TGA GCA TTC ATCAGG-3¢. The internal primers were 5¢-GCC AGC CAGCAA TAC GCC GAA-3¢ and 5¢-TTC GGC GTA TTGCTG ... H117Q. Mutant proteins were puriﬁed byNi-NTA afﬁnity matrix chromatography and assayed for enzymatic activity. All the mutants showed similar phos-photransferase activity as that of native Ndk...

Báo cáo khoa học: ADPase activity of recombinantly expressed thermotolerant ATPases may be caused by ppt

... chromatographyand analysis by MS (MALDI and, separately, LC ⁄ MS)identiﬁed AK as a contaminant that could cause theapparent ADP hydrolysis activity when coupled withthe ATPase activity of NtrC1 ... (S-frac-tion, 30 537.5 ± 6 Da; Q-fraction, 30 537.0 ± 6 Da).However, the S-fraction lost apparent ADPase activity and the Q-fraction had an elevated apparent ADPase activity. [Note that this cation ... of Technology, Chicago, IL, USAATPases associated with various cellular activities(AAA+ ATPases) form a large family of chaperone-likeproteins that use ATP hydrolysis to remodel numerousmacromolecular...

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