báo cáo khoa học: "Optimization of DNA delivery by three classes of hybrid nanoparticle/DNA complexes" pptx
báo cáo khoa học: "Optimization of DNA delivery by three classes of hybrid nanoparticle/DNA complexes" pptx
... Optimization of DNA delivery by three classes of hybrid nanoparticle /DNA complexes. Journal of Nanobiotechnology 2010 8:6.Submit your next manuscript to BioMed Centraland take full advantage of: • ... encap-sulated DNA (in)(Figure2).Optimization of NP /DNA complex delivery conditions to293 cellsWe compared the efficiency of DNA delivery to 293cells by the six NP /DNA complexes vs. a Lip2000 /DNA mixture. ... other hand, DNA encap-sulated NPs (in) are slower to release DNA and areprobably affected by biodegradation of the NPs withincells.Study of gene delivery with hybrid nanoparticle /DNA complexes...
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Báo cáo khoa học: "The ORF59 DNA polymerase processivity factor homologs of Old World primate RV2 rhadinoviruses are highly conserved nuclear antigens expressed in differentiated epithelium in infected macaques" pps
... ORF59 homologs of members of the RV1 and RV2 lineages of KSHV-like rhadinovirusesfrom chimpanzee and three species of macaques. Weobtained the complete sequences of the ORF59 homologs of the RV1 ... amount of cell-free RRV DNA present in theculture medium increased by nearly four logs in both cellcultures. By day 8, the cell-associated RRV DNA in theinfected RPFF cultures had increased by ... localization of ORF59 drivesnuclear import of the KSHV DNA polymerase during acti-vation of the replicative cycle of the virus [20]. Our in vitroresults demonstrate a strong correlation of the expressionand...
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Báo cáo khoa học: Increased NADPH concentration obtained by metabolic engineering of the pentose phosphate pathway in Aspergillus niger docx
... nonoxidative part of the PPPtwo out of three reactions are catalysed by transketo-lase (TKT; EC 2.2.1.1). Overproduction of theseenzymes might lead to increased flux through the PPP.The level of NADPH ... been increased inEscherichia coli by overproduction of G6PDH or6PGDH [10] and in Ralstonia eutropha by overproduc-tion of G6PDH [11] or by overproduction of 6PGDHor TKT [12].Glucose 6-phosphate ... in the range of the CV of the NADPHdetermination.DiscussionObvious tendencies found by characterization of the wild-typeIn the exponential phase of the ammonium culturesall of the PPP enzyme...
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Báo cáo khoa học: "Finding Deceptive Opinion Spam by Any Stretch of the Imagination" pptx
... of reviews, irrespective of the TripAdvisor ranking.9It has been hypothesized that popular offerings are lesslikely to become targets of deceptive opinion spam, since therelative impact of ... reviews.To obtain a deeper understanding of the nature of deceptive opinion spam, we explore the relative util-ity of three potentially complementary framings of our problem. Specifically, we view ... representative of deceptive opinion spam in gen-eral. Moreover, they are potentially detectable via off-the-shelfplagiarism detection software.310Proceedings of the 49th Annual Meeting of the Association...
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Báo cáo khoa học: "Robust Word Sense Translation by EM Learning of Frame Semantics" docx
... Translation by EM Learning of Frame Semantics Pascale Fung and Benfeng Chen Human Language Technology Center Department of Electrical & Electronic Engineering University of Science & ... translation process of a statistical machine trans-lation system. Manual inspection of the contras-tive error analysis data from a state -of- the-art SMT system showed that around 20% of the er-ror ... number of frames that are aligned to over 150 of Chinese catego-ries, while only a small number of English frames align to relatively few Chinese categories. After EM iterations, the majority of...
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báo cáo khoa học: " Novel exon combinations generated by alternative splicing of gene fragments mobilized by a CACTA transposon in Glycine max" pot
... transcripts hybridizing toa soybean FPK cDNA clone (Gm-c1023-5325) of a sizesimilar to those of the chimeric transcripts and that weremore highly expressed in cotyledons of all three isolinesthan ... cDNA derived amino acid sequences and openreading frames). Of interest were two putative chimericorfs present in several of the wp-cDNAs. One was com-posed of approximately 210 bp (70 aa) of ... aa of the UP intron plus the UP and CDC2 Exons. E (105 aa) with 34 aa of the FPK/MDH Intron plus 71 aa of the FPK Exon; F (105 aa) had 19 aa of FPK Intron, 77 aa of FPK Exon and 9 aa of MDH...
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Báo cáo khoa học: "Similarity between Words Computed by Spreading Activation on an English Dictionary" pptx
... T) of the node referred by the k-th link of sij. R[(T) is weighted activity of the nodes referred in the r6f~r~ rl of P/: R~(T) = ~ t~t .a~k(T), where t~k is thickness of the/~-th link ofri, ... = 0.596803 , er (of, of) = 0.045256. 4.3 Similarity of Extra Words The similarity of words in LDV and their derivations is measured directly on Paradigme; the similarity of extra words is ... Gloss~me A Closed Subsystem of English A dictionary is a closed paraphrasing system of nat- ural language. Each of its headwords is defined by a phrase which is composed of the headwords and their...
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Báo cáo khoa học: "Optimization of in situ hybridization assay using nonradioactive DNA probes for the detection of canine erpesvirus (CHV) in paraffin-embedded sections" pdf
... in situ hybridization for CHV 73In this study, several ISH protocols, which were consisted of the changes of enzyme-concentrations, the time of hybridization and hybridization probes, ... pre-treatment, and overnighthybridization. The practicality of digoxigenin-labeled probeis better than those of biotin-labeled probe in thehybridization assay for the detection of CHV. These resultssuggest ... 71–73Optimization of in situ hybridization assay using non-radioactive DNA probes for the detection of canine herpesvirus (CHV) inparaffin-embedded sectionsOkjin Kim*Department of Laboratory...
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Tài liệu Báo cáo khoa học: Steady-state and time-resolved fluorescence studies of conformational changes induced by cyclic AMP and DNA binding to cyclic AMP receptor protein from Escherichia coli ppt
... decreases by about 8 A˚to 18.7 A˚onbinding of cAMP to the anti-cAMP-binding sites of theprotein. The distance between the sulfur atom of Cys178 andthe C9–C10 bond of the indole ring of Trp85, ... the movement of the C-terminal domain of CRP by % 8A˚towards theN-terminal domain, which in consequence leads torearrangement of DNA- binding domains and cAMP-binding domains of the protein. ... of the CAP DNA complex at 2.5 A˚resolution: a complete picture of the protein– DNA interface. J. Mol. Biol. 260, 395–408.6. Passner, J.M. & Steitz, T.A. (1997) The structure of a CAP DNA complex...
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Báo cáo khoa học: Optimization of P1–P3 groups in symmetric and asymmetric HIV-1 protease inhibitors pptx
... replaced by co-ordination of a water molecule. This water molecule isco-ordinated by the NH group of the inhibitor, O of Gly27, and N of Asp29. The close packing between thechlorine atom and Cd of ... group is shifted upward by 1.5 A˚on one of thesides leading to loss of the interaction with O of Gly127 butinstead making contact with C and O of Gly149.Optimization of the P2/P2¢ side chainsThe ... fluorine-substituted benzyl group of compound6 created a P2 group that was too large, as reflected by thehigh Kivalue of 4.4 nM. Because of steric hindrance by themain-chain carbonyl group of Gly48, the Cl...
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