... cDNAs of human CB2and adenylyl cyclase type V (for adenylyl cyclase). The classicalpotent cannabinoid agonist, HU210, was used for the assays of binding affinity and adenylyl cyclase. The assays ... Bouaboula, M., Poinot-Chazel, C., Marchand, J.,Canat, X., Bourrie, B., Rinaldi-Carmona, M., Calandra,B.,LeFur,G.,andCasellas,P.Signaling pathwayassociated with stimulation of CB2 peripheral ... wereassayed for AC activity as described below. Transfectionefficiencies were normally in the range of 40-80%, as determined by staining for β-galactosidase activity.AC activityThe assay was...