... (CCTCAGAACGTTGATGGCA) and P2r (ATTGCTTTCCTTTTTCACAA GA) and allele-specific primer s Pnf (AGCATTTGGTTTTAAATTATG-GAGTATATG) and Pmr (GTTTTACTTACTCTCGTCTCCACAAAA). The PCR was run for 35 cycles ... K, Kameda T, Takenaka K, Oku S,Abe H, Katayose KS, Kubuki Y, Kusumoto K, Hasuike S, Tahara Y, Nagata K,Matsuda T, Ohshima K, Harada M, Shimoda K: Development of ET, primarymyelofibrosis and ... positivity at a very early stage and should have major implications in diagnosis and prevention of MPNs and other diseases that ma y beaffected by JAK2V617F.MethodsSample collection and DNA extractionDe-identified...