... Tsuda H, Shimizu CS, Taketomi MK, Hasegawa MM,Hamada A, Kawata KM, Inui N. Acrylamide; induction of DNA damage, chromosomal aberrations and celltransformation without gene mutations. Mutagenesis ... sodium azide (Sigma-Aldrich, USA) at a concentration of 1.5 µg/plate for TA 100 and TA 1535strains, and ICR 191 at a concentration of 0.1 µg/plate forTA 1537 strain were used.The microsomal fraction ... in 4 ml of fixative (3 : 1, methanol:glacialacetic acid) and washed 3 times with the fixative. After lastremoval of the fixative, a small portion of fixative wasadded and the pellet was resuspended....