Antibody Phage Display Methods and Protocols - part 8 docx

... Molecular Biology, vol. 1 78: Antibody Phage Display: Methods and Protocols Edited by: P. M. O’Brien and R. Aitken © Humana Press Inc., Totowa, NJ 28 Generation of Bispecifi c and Tandem DiabodiesSergey ... to Modify Immunoglobulins 313303From: Methods in Molecular Biology, vol. 1 78: Antibody Phage Display: Methods and Protocols Edited by: P. M. O’Brien and R. Aitken © Humana Press Inc., Totowa, ... tumor-targeting specifi city (Fig. 1). Smaller Ab-derived molecules include enzymatically produced 50-kDa Fabs and engineered 25-kDa single-chain Fvs (scFvs) consisting of heavy- and light-chain...

... 2,2′-di-p-nitrophenyl-5,5′,-diphenyl-3,3 -[ 3,3′-dimethoxy-4,4′-diphenylene] ditetrazolium chloride and 0. 38 mM5-bromo-4-chloro-3-indoxyl phosphate mono-(p-toluidinium) salt in 0.1 M Tris-HCl, ... (5) and (6) unbound free and labeled hapten removed by washing. 187 From: Methods in Molecular Biology, vol. 1 78: Antibody Phage Display: Methods and Protocols Edited by: P. M. O’Brien and R. ... bacteriophages. Folding Design 3, 321–3 28. 6. Hayhurst, A. and Harris, W. J. (1999) Escherichia coli Skp chaperone co-expression improves solubility and phage display of single chain antibody...

... Methods in Molecular Biology, vol. 1 78: Antibody Phage Display: Methods and Protocols Edited by: P. M. O’Brien and R. Aitken © Humana Press Inc., Totowa, NJ 18 Selecting Antibodies to Cell-Surface ... Molecular Biology, vol. 1 78: Antibody Phage Display: Methods and Protocols Edited by: P. M. O’Brien and R. Aitken © Humana Press Inc., Totowa, NJ16Proximity-Guided (ProxiMol) Antibody SelectionJane ... 2 7-9 41 1-0 1); fl uorescein-isothiocyanate-conjugated anti-sheep Ab; anti-M13 Ab (Pharmacia Biotech cat. no. 2 7-9 41 0-0 1). 15. Tetramethyl-benzidine dihydrochloride (TMB) solution (Sigma, T8665);...

... Biol. Chem. 2 68, 5302–53 08. 85 . He, M. and Taussig, M. J. (1997) Antibody- ribosome-mRNA (ARM) complexes as effi cient selection particles for in vitro display and evolution of antibody combining ... agarose gels and from solution. 8. Appropriate phagemid vector (e.g., MC03) (Fig. 1). 9. Low-melting-temperature agarose. 10. Long-wave, hand-held UV lamp. 11. UV-transparent shrink-wrap fi lm. ... libraries, in which light-chain (LC) and heavy-chain (HC) variable-region genes are cloned into a phagemid vector and subsequently displayed on the surface of the filamentous phage particle, have been...

... TC-3′HuVλ6-Back-APA 5′-ACC GCC TCC ACC AGT GCA CTT AAT TTT ATG CTG ACT CAG CCC CA-3′HuVλ7 / 8- Back-APA 5′-ACC GCC TCC ACC AGT GCA CAG RCT GTG GTG ACY CAG GAG CC-3′HuVλ9-Back-APA 5′-ACC ... CC-3′HuVλ3B-Back-APA 5′-ACC GCC TCC ACC AGT GCA CTT TCT TCT GAG CTG ACT CAG GAC CC-3′HuVλ4-Back-APA 5′-ACC GCC TCC ACC AGT GCA CAC GTT ATA CTG ACT CAA CCG CC-3′HuVλ5-Back-APA 5′-ACC ... TCA GG-3′VH ForwardHuJH1/2-For 5′-TGA GGA GAC GGT GAC CAG GGT GCC-3′HuJH3-For 5′-TGA AGA GAC GGT GAC CAT TGT CCC-3′HuJH4/5-For 5′-TGA GGA GAC GGT GAC CAG GGT TCC-3′HuJH6-For 5′-TGA...