... UÆmL )1 ) for30 min.RT-PCRTotal RNA was prepared with Trizol reagent (Invitrogen,Grand Island, NY) according to the manufacturer’s instruc-tions from near-confluent cultured fibroblasts. Preparation of ... 15 lgÆL )1 Æ24 )1 h) from the Division of Pediatric Endocrinology, Department of Pediatrics, KaplanMedical Center, Rehovot, Israel. All four ISS children had anormal increase of their abnormally ... of Biochemistry, School of Medicine,University of Patras, 2 611 0Rio-Patras, GreeceFax: +30 2 610 99 611 0Tel: +30 2 610 99 614 4E-mail: gpapavas@pat.forthnet.gr(Received 13 March 2006, revised 30 April2006,...