báo cáo khoa học: "Modélisation dynamique de systèmes génétiques régulation L’induction de l’opéron lactose d’Escherichia coli étude qualitative et numérique du modèle" pot

Báo cáo khoa học: The role of evolutionarily conserved hydrophobic contacts in the quaternary structure stability of Escherichia coli serine hydroxymethyltransferase pptx

... constants of PLP binding equilibrium and kinetic parameters determined with wild-type and mutant eSHMTs. Para-meters are expressed as the mean ± SD determined by nonlinear least squares ﬁtting ... formation of the dimer induced byPLP binding is not accompanied by a complete recov-ery of the native tertiary structure.DiscussionThe decrease of hydrophobic contact area determinedby the mutations ... three residues areinvariant in SHMT [18]. The second polypeptide loop,comprising residues 258¢–264¢, is a very conservedregion which interacts with the phosphate moiety ofPLP. It is delimited...

Báo cáo khoa học: Stability and ﬁbril formation properties of human and ﬁsh transthyretin, and of the Escherichia coli transthyretin-related protein potx

... 98,14943–14948.31 Hammarstrom P, Jiang X, Hurshman AR, Powers ET & Kelly JW (2002) Sequence-dependent denaturationenergetics: a major determinant in amyloid diseasediversity. Proc Natl Acad Sci ... Umea˚University, Sweden2 Department of Medical Biochemistry and Biophysics, Umea˚University, Sweden3 Division of Cell Biology, Diabetes Research Centre, Linko¨ping University, SwedenTransthyretin (TTR) ... andﬁsh transthyretin, and of the Escherichia coli transthyretin-related proteinErik Lundberg1, Anders Olofsson2, Gunilla T. Westermark3and A. Elisabeth Sauer-Eriksson11 Department of Chemistry,...

Báo cáo khoa học: Selection of full-length IgGs by tandem display on ﬁlamentous phage particles and Escherichia coli ﬂuorescence-activated cell sorting screening doc

... directbinding method and a capture method were utilized todetermine kinetic parameters. Briefly, PA63 was coupled toa CM5 chip to an equivalent of 750 response units (RU)by using 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide ... andsynthetic complementarity-determining-region diversity.Nat Biotechnol 23, 344–348.17 Knappik A et al. (2000) Fully synthetic human combi-natorial antibody libraries (HuCAL) based on modularconsensus ... moderate-affinity clones (R17 and R12) withaffinities in the single-digit nanomolar range and veryrare binders with modest binding kinetics (R15 andR42) that otherwise could probably not be detected...

Báo cáo khoa học: Using directed evolution to improve the solubility of the C-terminal domain of Escherichia coli aminopeptidase P Implications for metal binding and protein stability pptx

... inability to bind metals.Metalloproteins can fold via metal-dependent ormetal-independent pathways [20,21]. They may bindmetal ions before polypeptide folding, after completeprotein folding, ... domain AMPP#2 has a 157amino acid deletion, AMPP#12 has a 212 amino acid deletion,AMPP#3-22 has a 157 amino acid deletion, and AMPP#4-3 hasa 172 amino acid deletion. Mutations are R166G, G270V, ... site residues canproduce a soluble fragment. The E406G mutation con-verts a metal-binding ligand to a residue that is unli-kely to bind metal. The G270V residue is located nextto a metal-binding...

Báo cáo khoa học: "Modélisation de la croissance des peuplements réguliers de hêtre : dynamique des hiérarchies sociales et facteurs de production" pptx

... MÉTHODESLes données sur lesquelles s’appuie cette étude proviennent du réseau français de placettes per-manentes de production pour le hêtre (Oswald,1971 et 1981; Oswald et ... et multi-plicativement, les effets de l’âge, de ladensité, de la dimension et du statut so-cial. La structure linéaire de l’équation faci-lite le traitement des deux ... simultanément, et de façon cohérente, les deux niveaux de l’arbre et du peuplement : la densité influeglobalement sur la production du peuple-ment, selon une loi de saturation, et...

Báo cáo khoa học: "Modélisation de la structure locale et simulation d’éclaircies dans un peuplement hétérogèn" docx

... l’effectif de la première classe est C1, lesrangs des arbres de la première classe vont de 1 à C1; sil’effectif de la seconde classe est C2, les rangs des arbres de la seconde classe vont de C1+1 ... laconstruction du modèle de structure viendra ensuite. Ellegénéralise celle développée dans [5] en ce qu’elle intro-duit la notion de densité locale et permet de simuler desrègles de décision ... surface) et d’autrepart de modifier le degré de concurrence des espèces pardes priorités de prélèvement différentes selon le type de composition du placeau. On distinguera trois types de placeau...

Báo cáo khoa học: "Modélisation mécanique des vibrations propres d’un arbre soumis aux vents, en fonction de sa morphologie M Fournier" ppt

... modélisation de la structure et une si-mulation numérique des balancementspropres en vue d’effectuer des tests de sensibilité et de décider des données mor-phologiques et rhéologiques ... permet de distinguer 2 types de branches insérées sur le tronc, et nous suggère de ne retenir que les plus significatives du point de vue de leur rigidité et de leur ... Laboratoire de rhéologie du bois de Bordeaux, UMR C123 du CNRS,BP 10, domaine de l’Hermitage, Pierroton, 33610 Cestas Gazinet;2 Laboratoire de mécanique des solides de l’École...

Tài liệu Báo cáo khoa học: Molecular cloning and characterization of soybean protein disulﬁde isomerase family proteins with nonclassic active center motifs pdf

... the oxidation of thiol residues on the syntheticpeptide and the oxidative refolding of reduced anddenatured RNase A. In addition, neither proteinreduced the disul de bond in insulin (Fig. 2C). ... family proteins have oxidativerefolding activity on unfolded polypeptides and ⁄ or theability to reduce disul de bonds [7,8]. To determinewhether GmPDIL-3a or GmPDIL-3b possesses theseactivities, ... GmP-DIL-3a and GmPDIL-3b were resistant to proteasetreatment in the absence of detergent, and weredegraded when detergent was added (Fig. 4B), suggest-ing that they are both luminal proteins.Generally,...

Báo cáo khoa học: Molecular cloning, expression and characterization of protein disulﬁde isomerase from Conus marmoreus pdf

... marmoreus,andC. textile. Identical or similar residues are shaded in black or gray. The potential N-terminal signal peptides areboxed; the thioredoxin active sites are underlined; and the ER-retention motif ... Chinese Academy of Sciences, Graduate School ofthe Chinese Academy of Sciences, Shanghai, ChinaIn eukaryotes, all proteins that travel along or residein the secretory pathway are folded in the ... m NaCl).Peptide synthesisConotoxins tx3a and Tx3.1 [14,15] were chemically synthe-sized by using the Fmoc method on an ABI 433 A peptidesynthesizer. The crude reduced peptides were puriﬁed...

Báo cáo khoa học: Discovery and characterization of a Coenzyme A disulﬁde reductase from Pyrococcus horikoshii Implications for the disulﬁde metabolism of anaerobic hyperthermophiles doc

... Thecentral metabolism of this organism uses an unusualNADPH-dependent sul de dehydrogenase which iscapable of both the NADPH-dependent reduction ofelemental sulfur and the NADP+-dependent oxida-tion ... 2.0Oxidase-FADd22 5.9aDetermined at 200 lm CoA-S-S-CoA,bdetermined at 100 l mNADPH,cdetermined at 100 lm FAD,ddetermined at 100 lMNADH.CoADR from P. horikoshii D. R. Harris et al.1192 FEBS ... disul de reducing equivalents.AbbreviationsCoADR, coenzyme A disul de reductase (EC# 1.8.1.14); pfCoADR, P. furiosus coenzyme A disul de reductase; phCoADR, P. horikoshiicoenzyme A disul de reductase;...

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