... Functions, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Ibaraki,Japan2 Research Institute of Genome-based Biofactory, National Institute of Advanced Industrial Science ... substrate, tamarind seed xyloglucan (Dainip-pon Sumitomo Pharma, Osaka, Japan), in 50 mm sodiumacetate buffer (pH 5.5) at 45 °C for 15 min. The bicinchoni-nate assay was used to quantify reducing ... similar domains, each consisting of a seven-bladed b-propeller. These are locatedtandemly in the N- and C-terminal halves of the poly-peptide, and are joined on each edge of the domain toform a...