báo cáo khoa học: "Séquences homologues à l’élément P chez des espèces de Drosophila du groupe obscura et chez Scaptomyza pallida (Drosophilidae) " doc

báo cáo khoa học: "Séquences homologues à l’élément P chez des espèces de Drosophila du groupe obscura et chez Scaptomyza pallida (Drosophilidae)." doc

... D. buscküZaprionus, Zaprionus collartiNoteSéquences homologues à l’élément P chez des espèces de Drosophila du groupe obscura et chez Scaptomyza pallida (Drosophilidae). D. ... l’élément P apparaît ainsi largementrépandu dans le groupe obscura, et plus précisément dans le sous -groupe obscura. D.helvetica étant la seule espèce du sous -groupe affinis ... D. pseudoobscura,l’intensité du signal est à la limite du pouvoir de résolution de la technique et nepermet pas de conclure.La présence de séquences homologues à l’élément...

Báo cáo khoa học: Characterization of a second proliferating cell nuclear antigen (PCNA2) from Drosophila melanogaster docx

... that stably expressed V5-tagged DmPCNA1 andFlag-tagged DmPCNA1, V5-tagged DmPCNA2 andABCFig. 2. Expression of Drosophila melanogaster PCNA2 (DmPCNA2) during Drosophila development. (A) Northern ... amodel of a ring-shaped, three-dimensionalstructure of DmPCNA2 (left panel) andDmPCNA1 (right panel): upper panel, backview; lower panel, side view. In the dia-grams for DmPCNA1, purple ... D2E2loop[10–12]. The biophysical role of this loop is stillunknown.Analysis of DmPCNA2 expression during Drosophila developmentIn Drosophila, DmPCNA1 is highly transcribed in pro-liferating...

Tài liệu Báo cáo khoa học: Characterization of a chemosensory protein (ASP3c) from honeybee (Apis mellifera L.) as a brood pheromone carrier pdf

... Deleage & Geourjon [47].Peptide mapping and disul de bridge assignmentIn order to determine the disul de bridge pairing, ASP3cwas digested by trypsin and the resulting peptides wereseparated ... found, despite the fact that threeputative trypsin cleavage sites exist within the peptide. Thefact that the peptide was not cleaved by trypsin supports thenotion of an internal disul de bond ... recombinant ASP3c protein was subjected to trypsindigestion, which was expected to cleave the polypeptidechain linked by a disul de bridge. The tryptic peptidemixture was separated by RPLC (not...

Báo cáo khoa học: Characterization of a cathepsin L-associated protein in Artemia and its relationship to the FAS-I family of cell adhesion proteins pot

... cathepsin L, catalytic subunit, monomer; CLP,cathepsin L-like protease, dimer; CLAP, cathepsin L-associatedprotein; PI-PLC, phosphatidylinositol-specific phospholipase C;GPI, glycosyl-phosphatidylinositol; ... buffer only g ave identical linearresponses with gel length. In a separate experiment, CLAPwas treated with phosphatidylinositol-specific phospho-lipase C (PI-PLC) (Sigma) prior to analysis ... purified by SDS/PAGE, transferred to a poly(vinylidene difluoride) mem-brane and sequenced by Edman degradation along withfive peptides obtained by Lys-C treatment of CLAP(Eastern Quebec Peptide...

Báo cáo khoa học: Sequences and structural organization of phospholipase A2 genes from Vipera aspis aspis, V. aspis zinnikeri and Vipera berus berus venom Identiﬁcation of the origin of a new viper population based on ammodytin I1 heterogeneity docx

... Laboratoire de Recherche et de De´veloppement:Pharmacologie des Re´gulations Neuroendocriniennes, Paris, France.Note: The nucleotide sequences reported in this paper have beendeposited in ... Choumet*Unite´ des Venins, Institut Pasteur, Paris, FranceWe used a PCR-based method to determine the genomicDNA sequences encoding phospholipases A2(PLA2s) fromthe venoms of Vipera aspis ... organization of phospholipase A2genes fromVipera aspis aspis,V. aspis zinnikeriandVipera berus berusvenomIdentiﬁcation of the origin of a new viper population based on ammodytin I1heterogeneityIsabelle...

báo cáo khoa học: "Characterization of a caleosin expressed during olive (Olea europaea L.) pollen ontogeny" pdf

... 13-HPOD:(9Z,11E,13S)-13-hydroperoxy octadeca-9,11-dienoic acid; OB: oil body; PBS:phosphate buffered saline; PCD: programmed cell death; PEG-MAL:polyethylene glycol-maleimide; PL: phospholipid; PMC: pollen mother ... wall precur-sors, in order to promote and regulate pollen develop-ment [1]. During anther development, the tapetumundergoes programmed cell death (PCD) and become alipoidal m ass that is deposited ... EDTA:ethylenediaminetetraacetic acid; EGTA: ethylene-glycol-bis(β-aminoethylether)-N, N, N’,N’-tetraacetic acid; ER: endoplasmic reticulum;HEPES: (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid); 13-HPOD:(9Z,11E,13S)-13-hydroperoxy...

Báo cáo khoa học: " Medication errors: a prospective cohort study of hand-written and computerised physician order entry in the intensive care unit"

... (0.9%)Drug needed but not given as not prescribed properly3 (4.2%) 5 (4.3%)Inappropriate/inadequate additional information on prescription to adequately administer the drug appropriately8 ... reproduction in any medium, provided the original work is properly cited.AbstractIntroduction The study aimed to compare the impact ofcomputerised physician order entry (CPOE) without decisionsupport ... hospital protocols, and can prevent the prescription ofcertain drugs, thus implementing evidence based medicine [4]and improving clinical practice [5-7]. This prospective studycompares HWP...

Tài liệu Báo cáo khoa học: Modulation of a-synuclein aggregation by dopamine in the presence of MPTP and its metabolite pptx

... case of MPTP-modulated a-synuclein ﬁbrillationdescribed here, MPTP is still entrapped in the residualpellet which is not solubilized by urea.Effect of dopamine on MPTP and MPP+inducedchanges ... ofa-synuclein alone and in the presence of MPTP ⁄ MPP+is different.AbbreviationsMPP, 1-methyl-4-phenylpyridinum; MPTP, 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine; ThT, thioﬂavin T.1688 ... 2s.Chromatographic analysisRP-HPLC analysis of the samples was carried out to deter-mine the residual amounts of MPTP and MPP+in theaggregated samples. After completion of aggregation, thesamples...

Tài liệu Báo cáo khoa học: NirF is a periplasmic protein that binds d1 heme as part of its essential role in d1 heme biogenesis pdf

... NovagenpET-22b-NirF-strepII P. pantotrophus nirF cloned into pEG276 with strep II tag This workpET-22b -NirFH41A P. pantotrophus nirFH41Acloned into pET-22b This workpET-22b -NirFH41K P. pantotrophus ... pantotrophus nirFH41Kcloned into pET-22b This workpET-22b -NirFH41M P. pantotrophus nirFH41Mcloned into pET-22b This workpET-22b -NirFH41C P. pantotrophus nirFH41Ccloned into pET-22b ... This workpET-22b -NirFD129A P. pantotrophus nirFD129Acloned into pET-22b This workpET-22b -NirFD129Q P. pantotrophus nirFD129Qcloned into pET-22b This workS. Bali et al. Periplasmic NirF...

Tài liệu Báo cáo khoa học: Steﬁn A displaces the occluding loop of cathepsin B only by as much as required to bind to the active site cleft doc

... structures of DPPI alone andin complex with the inhibitor Gly-Phe-CHN2 [18]. Theamino peptidase cathepsin H has a covalently attachedstretch of eight residues originating from the propep-tide, termed ... Unligandedcathepsin B (PDB code: 1HUC) [11]. (B) Pro-peptide in dark blue (PDB code: 3PBH) [22].(C) Complex with stefin A, with stefin A ingreen. (D) A complex with chagasin (shownin cyan) (PDB code: 3CBJ) [25].M. ... screwcomponent of 15.38 A˚indicates a deviation from the idealscrew shift. When the cathepsin B molecules superimposi-tion parameters were applied on stefin A molecules, theirsuperimposition...

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