Báo cáo y học: "Advice and dissent" doc
Báo cáo y học: "Advice and dissent" doc
... constantly told, acquire and retain power by deceit and salesmanship, and frequently are contemptuous of the people they profess to serve. But, true as that cliché might be (and happily there ... Martin Rees, the President of the Royal Society, sent the government a set of guidelines that they say “would enhance confidence in the scientific advisory system and help government to secure ... on how to classify the dangers of cannabis (marijuana), ecstasy, and other drugs of abuse. On 30 October 2009, David Nutt was summarily fired by the British Home Secretary, Alan Johnson, for...
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Tài liệu Báo cáo Y học: DNA and RNA damage by Cu(II)-amikacin complex docx
... hipermodifiedbase Y3 7 (wybutine) (Figs 6 and 7). Also other amino-glycosides, as kanamycin A, neomycin B and a syntheticneomycin-neomycin dimer have been shown to inducefragmentation of tRNAPheat Y3 7 and ... 617–618.34. Yanagawa, H., Ogawa, Y. & Ueno, M. (1992) Redox ribonu-cleosides. Isolation and characterization of 5-ydroxyuridine,8-hydroxyguanosine & 8-hydroxyadenosine from Torula yeastRNA. ... [13] and also with cytoplasmic membrane [14,15]. Aminoglycosidesalso have severe toxic side-effects, on kidney and inner ear[16,17]. Fe(II) complexation and generation of hydroxylradicals by...
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Tài liệu Báo cáo Y học: Human and Drosophila UDP-galactose transporters transport UDP-N-acetylgalactosamine in addition to UDP-galactose doc
... O76865 and O9W4W6) and i ndicated by the arrowheads. The s ymbol ÔVÕ indicates a po ten tial N -glycosylation site. A putative polyadenylation signal is enclosed by a box. (B) Hydrophobicity plot ... mMphenylmethanesulfonyl¯uoride], and thesamples were fractionated b y electrophoresis on a 12%SDS/polyacrylamide gel. The separated polypeptides wereelectotransferred to a poly(vinylydene ... secretory and membrane-boundglycoproteins and glycolipids p lay important roles invarious biological processes. Two major groups of proteins,nucleotide sugar transporters (NSTs) and glycosyltransfe-rases,...
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Tài liệu Báo cáo Y học: Expression and characterization of active site mutants of hevamine, a chitinase from the rubber tree Hevea brasiliensis docx
... structural and enzyme kinetic studies. Mutation of Tyr183 t o phenylalanineproduced an enzym e with a lower kcat and a slightly higherKmthan the wild-type enzyme. Mutating Asp125 and Glu127 ... (1995) Stereochemistry of chitinhydrolysis by a plant chitinase/lysozyme and X-ray structure of acomplex with allosamidin: evidence for substrate assisted catalysis.Biochemistry 34, 15619–15623.14. ... the transitionstate by hydrogen bonding to the )1 N-acetyl carbonyloxygen atom. This h ydrogen bond stabilizes the partiallynegative charge on the carbonyl oxygen, thereby facilitatingFig....
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Tài liệu Báo cáo Y học: Mutations in the docking site for cytochrome c on the Paracoccus heme aa3 oxidase Electron entry and kinetic phases of the reaction pptx
... two-subunitenzyme (Fig. 2) and all mutants in positions 121 and 122(W121F, W12 1Y, W121Q /Y1 22Q, W121F /Y1 22F, W121G and W12 1Y/ Y122W) were decreased to 36 mM.Oftheremaining mutants, two showed wild-type ... analyzedby SDS/PAGE to verify their subunit composition, con-centrated and stored at )80 °C.Steady-state kinetics and determination of the ionicstrength dependenceCytochrome oxidase activity ... were re-analyzed side by side, and are presented for a complete overview.bKmvalue taken from the high-affinity and kcatfrom the low-affinity phase.cPre-steady-state kinetics of cytochrome...
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Tài liệu Báo cáo Y học: Characterization and regulation of yeast Ca2+-dependent phosphatidylethanolamine-phospholipase D activity docx
... Saccharomyces cerevisiae which isCa2+-dependent, preferentially hydrolyses phosphatidyl-ethanolamine (PtdEtn) and phosphatidylserine and does notcatalyse a transphosphatidylation with primary short-chainalcohols. ... catalytic proper-ties. Its activity is Ca2+-dependent; it preferentiallyhydrolyses phosphatidylethanolamine (PtdEtn) and phos-phatidylserine (PtdSer); and its activity is not stimulated byPtdInsP2. ... phosphate synthase [32]. It is therefore highly likely thatyeast PtdEtn-PLD is encoded by a non-HKD gene and maythus represent a novel PLD gene family. A prokaryoticPLD activity similar to yeast...
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Báo cáo Y học: Isolation and characterization of a thioredoxin-dependent peroxidase from Chlamydomonas reinhardtii doc
... tohomogeneity by HPLC and digested with t rypsin. Thetryptic peptides were separated by HPLC and some of themwere totally or partially analyzed by Edman sequencing and/ or by MALDI-TOF mass spectrometry ... 2Cys-Prx enzymes previously described i nyeast, mammals and plants, Ch-Prx1 d isplayed antioxidant and peroxidase activities. The enzyme could reduce hydro-gen peroxide as well as alkyl hydrogen ... containtwo and one conserved cysteines, respectively. 2Cys-Prxproteins are reduced by the AhpF protein in bacteria, and by the thioredoxin/thioredoxin reductase system in yeast and animals, w hile 1Cys-Prx...
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Báo cáo Y học: Structural and biochemical characterization of calhepatin, an S100-like calcium-binding protein from the liver of lungfish (Lepidosiren paradoxa) docx
... Sequence analysis and molecular modellingpredict the two EF-hand motifs characteristic of theS100 family. Metal-binding properties were studied by adirect45Ca2+-binding assay and by fluorescence ... SDS/PAGE analysis, only lungfish liver and intestine showed the calhepatin band (data notshown).Fig. 3. Primary structure of calhepatin and its sequence alignment with S100 family members. Peptide ... tissue-specific and cell-specific expression [4]. Some members of thefamily also bind Zn2+ and/ or Cu2+[5]. Most S100proteins can form noncovalent dimers by a symmetrichomodimeric fold mediated by hydrophobic...
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Báo cáo Y học: Expression and characterization of recombinant vitamin K-dependent c-glutamyl carboxylase from an invertebrate, Conus textile doc
... carboxylase activity. The effect ofproFIX18, proPT18 and pro-e-TxIX/12 on FLEEL carboxylation bythe recombinant bovine carboxylase (closed symbols) and the recom-binant Conus carboxylase (open symbols) ... onConuscarboxylase activityOur work [43] and that of Bandyopadhyay et al.[42]demonstrated the importance of the propeptide in directingc-carboxylation of Conus precursor substrates by the Conuscarboxylase. ... K-dependent carboxylase: effect of ammonium sulfate onsubstrate carboxylation and on inhibition by stereospific substrateanalogs. Biochim. Biophys. Acta 1034, 11–16.51. Bandyopadhyay, P.K., Garrett,...
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Báo cáo Y học: Unfolding and refolding studies of frutalin, a tetrameric D-galactose binding lectin doc
... & Wolynes, P.G.(1995) Funnels, pathways and the energy landscape of proteinfolding: a synthesis. Proteins 21, 167–195.2. Dill, K.A. & Chan, H.S. (1997) From Levinthal to pathways tofunnels. ... Schimmel, P.R. (1980) Biophysical Chemistry.Part II: Techniques for the Study of Biological Structure and Function: Spectroscopic Analysis of Biopolymers. W. H. Freeman,New York, USA.19. Perczel, ... for Nfrutalin and 348 nm for Ufrutalin, and Rfrutalin was closely similar to Nfrutalin for both kemissmax and intensity. The kemissmaxof Mfrutalin was 353 nm and theintensity was similar...
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