Protein family review The annexins docx

... establishes the invertebrate ancestry of vertebrate annexins, none of the 12 annexins in the vertebrate A family have (yet) been assigned atrue invertebrate ortholog. The vertebrate A family includes the ... representative annexin proteins. Orthologs of the 12 human annexins shown in other vertebrates have the same structures, withstrict conservation of the four repeats in the core region (black) ... sequence in the amino-terminal regions (shaded). HumanANXA1 and ANXA2 are shown as dimers, with the member of the S100 protein family that they interact with. Domain structures for other modelorganisms...

Báo cáo khoa học: The astacin protein family in Caenorhabditis elegans docx

... metalloproteases. The first evidence for the existence of the astacin protein family can be traced back to the year 1967, when one of us(R. Zwilling) observed a proteolytic activity in the digestivefluid of the ... sequences down to the last30–50 codons of the astacin genes was fused in frame to the reporter gene GFP. Thus, the intergenic region as well as the protein coding regions of the astacin-like ... innine genes (Fig. 2). The missing of signal peptides in thesegenes may reflect specific intracellular functions of non-secreted proteins. On the other hand the lack of these signalpeptides could...

Protein family review docx

... featuresCurrently, the structural features of this family of proteinsare poorly defined, but information gleaned from the protein sequence has enabled some predictions of structure andmotifs within the proteins. ... the cytoplasmic protein coat of caveolae, making Cav-1 the firsttrue protein marker of caveolae [2]. Subsequent cloning of the Cav-1 cDNA revealed that it was identical to another protein, VIP21, ... Cav-2Genome Biology 2004, 5:214commentreviewsreports deposited researchinteractionsinformationrefereed research Protein family review The caveolin proteinsTerence M Williams*†and Michael...

Báo cáo y học: "The identification and characterization of a novel protein, c19orf10, in the synovium" docx

... alternative splicing, and protein sequence of c19orf10. (a) The chromosomal localization of the region containing the c19orf10 gene is indicated on the ideogram. The area containing the c19orf10 gene ... in other cases, there is anapparent absence of c19orf10 staining (Figure 4f,h). Theseobservations raise the possibility that there may be either func-tional or compositional changes in the ... citation purposes) the MS data. WE and KS provided instrumentation. HE-G per-formed immunohistology and reviewed the manuscript. JAWinitiated the study, planned the studies, and revised the manu-script....

Tài liệu Báo cáo khoa học: Cobalamin uptake and reactivation occurs through speciﬁc protein interactions in the methionine synthase–methionine synthase reductase complex docx

... inset). The absorptionspectrum of the puriﬁed enzyme was typical of the hydroxycobalamin form of the enzyme (Fig. 2). The recovery of the activity was  10%, and the enzymewas puriﬁed 3669-fold. The ... footnote) in the reactionmixture may reduce a proportion of the FMN domain,converting some of the enzyme to the active form. The  3-fold increase in activity may arise from the bindingof the FMN ... present in the assay may be reducing FMNand ⁄ or the cobalamin. The source of the reducing agent isunknown, but it may originate from dithionite on the surface of the gloves in the anaerobic...

Tài liệu Báo cáo khoa học: Speciﬁc membrane binding of the carboxypeptidase Y inhibitor IC, a phosphatidylethanolamine-binding protein family member doc

... available regard-ing the phospholipid and membrane binding of the other members of the PEBP family. Consequently, the binding characteristics of PEBP proteins, includingthose of the binding of ICto ... centrifugationof the samples, proteins bound to liposomes were analyzed bySDS ⁄ PAGE of the resulting pellets. (C) The amounts of ICin the pellets. The amounts of ICwere quantitated with the UN-SCAN-IT ... located in the N-terminal seven resi-dues, the low afﬁnity of d1–7ICcould be caused by the conformational change in the vicinity of the ﬁve basicresidues in this mutant protein. The present...

Tài liệu RESULTS BASED MANAGEMENT IN THE DEVELOPMENT CO-OPERATION AGENCIES: A REVIEW OF EXPERIENCE docx

... Canada, the Office of the Auditor Generaland the Treasury Board Secretariat have been the primary promoters of reforms across the federal government.While there have been variations in the reform ... isthat the indicators provide managers with the information they need to do their job.10 While on the one hand,indicator data should be of sufficient quality to be credible and ensure the right ... policy-makers in the partnercountry government or in the donor agency, as well as the donor country’s parliament and taxpayers.Within the implementing agency, as the level of management changes, the level...

Tài liệu Báo cáo Y học: A family of expressed antifreeze protein genes from the moth, Choristoneura fumiferana ppt

... freezing.During the winter they also increase the concentration of the cryoprotectant, glycerol, 10-fold and desiccate to 40%of the prediapause water content [4]. Taken together,these adaptations ... least some of the  17 genes of the family are spacedseveral kb ap art (as fo r Afp-Lu1 and 2 .7a), e ven though theymay be linked.In both Afp-Lu1 an d Afp-Iu1, the majority of the sequence ... regions. The dashed horizontal line for the gene2.7a ind icate s the putative 3¢ UTR o f the ge ne, b ased on the t ranscriptsize estimation. The white box in the 3¢ end of Afp-Lu1 is a 517-nucleotide...

Tài liệu Báo cáo Y học: Analyses of the CYP11B gene family in the guinea pig suggest the existence of a primordial CYP11B gene with aldosterone synthase activity docx

... Thus, the proteins of the CYP11Bsubfamily catalysing the last biosynthetic steps are the keyenzymes for the synthesis of both mineralocorticoids andglucocorticoids.From molecular cloning of the ... wecompared protein sequences of the CYP11B family. Theseanalyses showed that CYP11B2 proteins are more closelyrelated to each other than CYP11B1 proteins across species.Furthermore, the 3¢ UTR of the ... evolutionand function of the hormonal system. We ﬁrst cloned the genes of the CYP11B family of the guinea pig. The 11b-hydroxylase of the guinea pig showed higher substratespeciﬁcity than the aldosterone...

Báo cáo khoa học: Muramyl-dipeptide-induced mitochondrial proton leak in macrophages is associated with upregulation of uncoupling protein 2 and the production of reactive oxygen and reactive nitrogen species docx

... [28]. These findings agree with the hypothesis [29] that an increase in the mitochondrialmembrane potential would slow the transport of elec-trons through the respiratory chain, increasing the ... upregulates the expres-sion of the mitochondrial protein uncoupling protein 2 (UCP2), and pre-treatment with vitamin E attenuates upregulation of UCP2. These findingssuggest that the MDP-induced ... confirmed the effects ofMDP in vitro on murine macrophages. The macrophage system was thenused to analyse the mechanism of the MDP-induced mitochondrial protonleak. Our results demonstrate that the...

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