... candidates that can be shortlistedwhen setting up a new experimental design. Therefore, wedetermined the expression of previously tested referencegenes in a setting of virus infected human cell ... probes. This is the great advantage of the ∆∆CTmethod, or any other method which directly comparespaired samples. From this point of view the use of amethod like the ∆∆CT should be applied first ... Biotechnol Lett 2004, 26:509-515.7. Vandesompele J, De Preter K, Pattyn F, Poppe B, Van Roy N, DePaepe A, Speleman F: Accurate normalization of real-timequantitative RT-PCR data by geometric averaging...