Báo cáo khoa hoc:"Association of a missense mutation in the bovine leptin gene with carcass fat content and leptin mRNA levels" pps
Báo cáo khoa hoc:"Association of a missense mutation in the bovine leptin gene with carcass fat content and leptin mRNA levels" pps
... 10.1051/gse:2001006Original articleAssociation of a missense mutation in the bovine leptin gene with carcass fat content and leptin mRNA levelsFiona C. BUCHANAN a, ∗, Carolyn J. FITZSIMMONSb,Andrew G. VANKESSEL a , ... correlated with levels of carcass fat. The T allele was associated with fatter carcasses and the C allele with leanercarcasses. The frequencies of the SNP alleles among breeds indicated that British ... residual error. The results from the RPA were analyzed using a one-way ANOVA. Leptin SNP correlated with fat and mRNA levels 115amplification, cloning, partial sequence analysis, and use as loading...
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Báo cáo khoa hoc:" Role of metabolically active hormones in the insulin resistance associated with short-term glucocorticoid treatment" doc
... combination of fasting insulin and triglycerides [14], 'insulin sensitivityindex' (ISI), was also used as surrogate a marker of insulinsensitivity.Power calculation and statistical analysisWe ... were rand-omized to dexamethasone and 12 to placebo. Baselineplasma TNFα, ghrelin, leptin and adiponectin were com-parable among subjects in the dexamethasone and pla-cebo groups before intervention ... log-trans-formed prior to this). Data were analysed usingparametric and non-parametric tests, with ANOVA and multiple linear and logistic regression analyses as appro-priate (SPSS Inc., Chicago,...
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Báo cáo khoa học: Contribution of a central proline in model amphipathic a-helical peptides to self-association, interaction with phospholipids, and antimicrobial mode of action ppt
... that the replacement of a Pro with Leu or Ala in the hybrid analog P18decreases its antibacterial activity and increases itshemolytic activity [39]. In general, introduction of a Pro near the ... of a Pro with an Ala maintained ordecreased the antimicrobial activity but significantlyincreased the hemolytic activity. In addition, Oh et al.[38] reported that a cecropin A magainin II hybrid ... isodichroicpoint at 203 nm, indicating a two-state equilibriumbetween a random coil and an a- helix. In purewater, M17 and M25 also became more a- helical as the NaCl concentration was increased (Fig....
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Báo cáo khoa học: Characterization of a prokaryotic haemerythrin from the methanotrophic bacterium Methylococcus capsulatus (Bath) ppt
... seven ligands of the diiron site in haemeryth-rins are amino acid residues each located within the four helices, and by diiron binding generating anintramolecular cross-linking of these helices, ... analysis modelled the putative haemerythrin to a four a- helix bundle, maintaining both the conservedhaemerythrin positioning of the amino acid residuescoordinating the metal ions, and the hydrophobicpocket ... chemoreceptor was proposed to have a mem-brane-spanning domain, placing the C-terminallylocated Hr domain in the cytoplasm. The biologicalfunction of DcrH and its Hr-like domain is not fullyelucidated.To...
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Báo cáo khoa học: Characterization of a hemocyte intracellular fatty acid-binding protein from crayfish (Pacifastacus leniusculus) and shrimp (Penaeus monodon) pdf
... [12].Vertebrate FABPs are involved in cellular fatty acidtransport and utilization and compartmentalization of intracellular fatty acid storage, and also in fatty acid-induced regulation of gene expression ... ligand-bindingstudies with recombinant msCRABP showed high affin-ity binding for fatty acids and negligible interaction with RA and other retinoids, and hence this proteinmay be a FABP rather ... lig-ands are bound. Binding of RA as well as of manyfatty acids requires interaction with the side chains of a characteristic triad of conserved amino-acid residues in the ligand-binding hollow, but...
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Báo cáo khoa học: Characterization of a digestive carboxypeptidase from the insect pest corn earworm (Helicoverpa armigera) with novel specificity towards C-terminal glutamate residues pptx
... present in the databases. The two proteins migratingat 50 and 55 kDa (bands A and B; Fig. 1A) wereidentified from their N-terminal sequences as similar to a- amylase (accession no. AAA17751) ... N-terminus of the proprotein in- frame and adjacent to the cleavage point of the yeast a- mating factor secretionsignal encoded by the vector. In addition, a (His)6-tagencoded by the vector was added ... digested, with anincrease in staining of the bands of 36 kDa and 13 kDa. On further incubation with trypsin the 13 kDapolypeptide is itself digested by trypsin, and decreases in amount until...
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Báo cáo khoa học: Inhibition of a-synuclein fibrillization by dopamine analogs via reaction with the amino groups of a-synuclein Implication for dopaminergic neurodegeneration pot
... (Markham Ontario,Canada). All other reagents were of analytical quality.Expression and purification of a- Syn and a- Syn1)60 a- Syn and a- Syn1)60genes were subcloned into the pET- 3a vector, and ... China2 Shanghai Institute of Materia Medica, Shanghai, China3 Shanghai Institute of Nuclear Research, Shanghai, China4 Bio-X Research Center, Shanghai Jiaotong University, Shanghai, ChinaParkinson’s ... of DA analogs react with the amino groups of a- Synchain, generating a- Syn–quinone adducts. It is likely that the amino groups of a- Syn undergo nucleophilic attack on the quinone moiety of DA...
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Báo cáo khoa học: Effects of a tryptophanyl substitution on the structure and antimicrobial activity of C-terminally truncated gaegurin 4 doc
... spectra of the GGN4 analogues, including the native GGN4,showed a strong negative band near 200 nm and a weak and broad band around 222 nm, indicating a predominantlyrandom-coil conformation with a ... wouldbe advantageous to facilitate the amphipathic interactionbetween the peptide and the membrane surface, as the tryptophan side chain is amphiphilic in nature. The tryptophan side chain conformation ... from the skin of variousamphibians, and six antimicrobial peptides, named gaegu-rins (GGNs), were also isolated from the skin of a Koreanfrog, Rana rugosa [14]. Some of them, particularly...
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Báo cáo khoa học: " Development of a sandwich ELISA for the detection of Listeria spp. using specific flagella antibodies" potx
... in ascites, and selected for developing a sandwich ELISA. The subclass of the MAb 2B1 and MAb 7A3 were identified as IgG1 and IgG 2a , respectively, with kappa light chain. The MAb 7A3 and ... RT. After addingABTS (KPL, USA) and 30 min’s incubation at RT,absorbance was measured at 405 nm.Sandwich ELISA The sandwich ELISA was performed on microtiter plates.Each well was coated with ... of a sandwich ELISA for the detection of Listeria spp. using specific flagella antibodies 43washing, 100µl of each HRP conjugated MAb was addedinto each well and incubation for 30 min at...
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Báo cáo y học: "Association of a specific haplotype across the genes MMP1 and MMP3 with radiographic joint destruction in rheumatoid arthritis" ppt
... was assessed by the Ratingen score [28], a modification of the Larsen score.It evaluates 38 joints separately (all proximal inter-phalangeal and metacarpophalangeal joints, four sites in the ... for each radiograph by division of the Ratingen score by the disease duration at the time that the radiograph was taken.DNA isolationBlood samples containing EDTA as anti-coagulant wereobtained ... section.RR and GH took care of all patients. They collectedclinical data and all blood samples and radiographs and scored them by means of the Ratingen score.UW carried out the HLA typing.BM-M...
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