... method to form a complex between TVA II and a pullulanmodel substrate by using partial hydrolyates of pullulan andan inactive TVA II mutant, D325N. A hexasaccharidecontaining two panose units, ... primer: 5¢-GATCACACTCTCGCGAAACAAATAATTCATCACCG-3¢. The underlined nucleotide in the primer indicates the mismatched nucleotide creating thealanine substitution mutation. DNA sequencing confirmedthe ... Yanase, M., Takata, H., Shimada, J.,Handa, S., Takada, T., Umeyama, H. & Okada, S. (1996) Con-trolling substrate preference and transglycosylation activity ofneopullulanase by manipulating steric...