... Germany4 Macromolecular Crystallography, BESSY GmbH, Berlin, Germany5 Institute of < /b> Chemistry and < /b> Biochemistry – Crystallography, Free University, Berlin, Germany6 Department of < /b> Structural Biology, ... morphology of < /b> the generatedfilament bundles.Calcium affinity of < /b> Iba1 and < /b> Iba2Homodimerization and < /b> actin binding of < /b> Iba1 and < /b> Iba2were similar in the absence or presence of < /b> calciumions. Calcium binding ... by a cycle of < /b> polymerization and < /b> depolymerization,isoform separation by hydroxyapatite chromatography, and< /b> a final gel filtration step.Actin cosedimentation and < /b> cross-linking assaysSamples of...