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Báo cáo y học: "Phenotypic and functional characterization of switch memory B cells from patients with oligoarticular juvenile idiopathic arthritis" pps

Báo cáo y học:

Báo cáo y học: "Phenotypic and functional characterization of switch memory B cells from patients with oligoarticular juvenile idiopathic arthritis" pps

... expansion of< /b> switch < /b> memory < /b> B cells and < /b> IgG-secreting plasma blasts in theSF from oligoarticular JIA patients. These memory < /b> B cells belonged to both the CD27+ and < /b> the CD27- subsets and< /b> expressed ... switch < /b> memory < /b> B cells from juvenile idiopathic arthritis (JIA) patientsChemokine receptor and < /b> CD86 expression on switch < /b> memory < /b> B cells from juvenile idiopathic arthritis (JIA) patients. Cells from ... CD19+CD27+ memory < /b> B cells have beenidentified: (a) IgM memory < /b> (CD19+CD27+IgG-IgA-), and < /b> (b) class switch < /b> memory < /b> (CD19+CD27+IgG+IgA+) B cells [14,16]. The percentage of < /b> IgM memory < /b> B cells...
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Báo cáo y học:

Báo cáo y học: "Phenotypic and functional characterisation of CD4+ memory T cells homing to the joints in juvenile idiopathic arthritis" pps

... expressed by several cell types (lym-phocytes, dendritic cells, B cells and < /b> plasma cells) [18,24],only areas characterised by a clear lymphocyte infiltration(as defined by anti-CD3 and < /b> anti-CD4 ... intensity. (a, b) Expression of < /b> IFN-γ (a) and < /b> CXCR3 (b) by SF CCR7+ and < /b> CCR7- memory < /b> CD4+ cells from 10 patients with juvenile idiopathic arthritis (JIA). Boxes contain values falling between ... CCR7- memory < /b> CD4+ cells from synovial fluidExpression of < /b> CXCR3 and < /b> interferon (IFN)-γ by (SF) CCR7+ and < /b> CCR7- memory < /b> CD4+ cells from synovial fluid. IFN-γ expression was investigated by three-colour...
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Báo cáo Y học: Structural and functional characterization of a C-type lectin-like antifreeze protein from rainbow smelt (Osmerus mordax) potx

Báo cáo Y học: Structural and functional characterization of a C-type lectin-like antifreeze protein from rainbow smelt (Osmerus mordax) potx

... digestions of < /b> deglycosylated and < /b> untreated smelt AFP in presence and < /b> absence of < /b> Ca2+. Both deglycosylated and < /b> untreated smeltAFP were incubated with protease in the presence or absence of < /b> 20 mMCa2+as ... Modulation of < /b> ligand-binding activity by Ca2+ and < /b> pH. J. Biol. Chem. 263,9752–9760.28. Varki, A. (1993) Biological roles of < /b> oligosaccharides: all of < /b> thetheories are correct. Glycobiology 3, 97–130.29. ... [19].Dimerization of < /b> smelt AFP is unlikely to increase activityby increased ice binding because only a dimer with perfectlyaligned ice-binding sites would have enhanced binding to ice and < /b> this is not likely...
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Tài liệu Báo cáo khoa học: Molecular and functional characterization of adenylate kinase 2 gene from Leishmania donovani pdf

Tài liệu Báo cáo khoa học: Molecular and functional characterization of adenylate kinase 2 gene from Leishmania donovani pdf

... performed with an anti-AKpolyclonal antibody obtained from previously immunizedrabbits. A single band of < /b> 26 kDa was detected, as shown inFig. 4B. Functional < /b> characterization < /b> of < /b> recombinant AKThe ... homogeneity. Kinetic analysis of < /b> purifiedL. donovani AK showed hyperbolic behaviour for both ATP and < /b> AMP, with Kmvalues of < /b> 104 and < /b> 74 lM, respectively.The maximum enzyme activity (Vmax) ... kinetic behaviour of < /b> L. donovani recombin-ant AK2 is hyperbolic for both AMP and < /b> ATP in theforward reaction. Like most AKs, the enzyme was stronglyinhibited by Ap5A, with Kivalues of < /b> the...
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Tài liệu Báo cáo Y học: Structural and biochemical characterization of neuronal calretinin domain I– II (residues 1– 100) Comparison to homologous calbindin D28k domain I–II (residues 1 –93) pdf

Tài liệu Báo cáo Y học: Structural and biochemical characterization of neuronal calretinin domain I– II (residues 1– 100) Comparison to homologous calbindin D28k domain I–II (residues 1 –93) pdf

... agrees with the large body of < /b> independent data collected on Calb and < /b> truncated Calbs by Kumar’s group. This latter workrevealed that EF-hands II and < /b> VI of < /b> Calb do not bindcalcium and < /b> that Calb binds ... Poland;2Department of < /b> Biochemistry and < /b> Molecular Biology, University of < /b> Debrecen, Hungary;3Department of < /b> Inorganic Chemistry, and< /b> 4Research Group for Antibiotics, Department of < /b> Chemistry, ... acrylamide gels and< /b> stained with Coomassie blue. Bovine S10 0B, bovinea-lactalbumin and < /b> horse skeletal myoglobin (all from Sigma) were used as control protein samples. Myoglobin and < /b> a-lactalbumin...
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Báo cáo khoa học: Molecular and functional characterization of novel CRFR1 isoforms from the skin pptx

Báo cáo khoa học: Molecular and functional characterization of novel CRFR1 isoforms from the skin pptx

... cotransformed by constructs containing CRE, CaRe, AP1, NF-jB and < /b> SRE elements and< /b> different CRFR1 isoforms. (A) Stimulation by CRF. (B) Stimulation by CRF, forskolin and < /b> PMA (TPA) and < /b> inhibition by PKA ... 5% fetal bovine serum, antibiotics and < /b> 0.5 mM3-isobutyl-1-methylxanthine (IBMX), and< /b> transfected COS cells were incubated with the ligand for1 h at 37 °Cand5%CO2in the incubator.Cyclic AMP ... inhibitedCRE activity. Significantly higher reporter gene expressionby CRF was observed than that mediated by 4b- phorbol12-myristate 13-acetate and < /b> forskolin alone, being compat-ible with...
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Báo cáo khoa học: Molecular and functional characterization of a novel splice variant of ANKHD1 that lacks the KH domain and its role in cell survival and apoptosis docx

Báo cáo khoa học: Molecular and functional characterization of a novel splice variant of ANKHD1 that lacks the KH domain and its role in cell survival and apoptosis docx

... using the yeast two-hybrid system as previouslydescribed [9]. A 915-bp fragment was initially identified and < /b> further confirmed through both repetitions of < /b> theyeast two-hybrid assay and < /b> using ... thatVBARP treatment is specific and < /b> does not alter theglobal cellular transcription. Effect of < /b> VBARP siRNAon cell viability was tested by the trypan blue exclusionassay and < /b> cell viability assay. ... Schafer1 and < /b> Velpandi Ayyavoo11 Department of < /b> Infectious Diseases & Microbiology, Graduate School of < /b> Public Health, University of < /b> Pittsburgh, PA, USA2 Health Sciences Library System, University...
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Báo cáo Y học: Structural and biochemical characterization of calhepatin, an S100-like calcium-binding protein from the liver of lungfish (Lepidosiren paradoxa) docx

Báo cáo Y học: Structural and biochemical characterization of calhepatin, an S100-like calcium-binding protein from the liver of lungfish (Lepidosiren paradoxa) docx

... characteristic of < /b> theS100 family. Metal-binding properties were studied by adirect45Ca2+-binding assay and < /b> by fluorescence titration.Calhepatin binds Ca2+ and < /b> Cu2+but not Zn2+.Cu2+binding ... with aletter (E for peptides derived by Glu-C digestion and < /b> K for peptides obtained by Lys-C digestion) and < /b> a number that agrees with that of < /b> Fig. 2.Numbers above the sequence indicate residue ... 7.4).45Ca2+binding was determined by the method of < /b> Mani and< /b> Kay[77]using15lMapo-(lungfish calhepatin) as describedby Dell’Angelica et al. [10]. Binding data were analyzed bynonlinear regression...
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Báo cáo khoa học: Structural and functional characterization of human Iba proteins ppt

Báo cáo khoa học: Structural and functional characterization of human Iba proteins ppt

... Germany4 Macromolecular Crystallography, BESSY GmbH, Berlin, Germany5 Institute of < /b> Chemistry and < /b> Biochemistry – Crystallography, Free University, Berlin, Germany6 Department of < /b> Structural Biology, ... morphology of < /b> the generatedfilament bundles.Calcium affinity of < /b> Iba1 and < /b> Iba2Homodimerization and < /b> actin binding of < /b> Iba1 and < /b> Iba2were similar in the absence or presence of < /b> calciumions. Calcium binding ... by a cycle of < /b> polymerization and < /b> depolymerization,isoform separation by hydroxyapatite chromatography, and< /b> a final gel filtration step.Actin cosedimentation and < /b> cross-linking assaysSamples of...
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Báo cáo khoa học: Cloning and functional characterization of Arabidopsis thaliana D-amino acid aminotransferase – D-aspartate behavior during germination pdf

Báo cáo khoa học: Cloning and functional characterization of Arabidopsis thaliana D-amino acid aminotransferase – D-aspartate behavior during germination pdf

... on black background, and < /b> conserved amino acid residues with high and < /b> low similarity are indi-cated by double dots and < /b> single dots, respectively. Amino acid residues plausibly involved in the binding ... reportdemonstrates broad substrate specificity, with d-Glu and < /b> d-Ala being high-affinity substrates. The variousd-amino acids found in most plants [37] may beformed by the activity of < /b> homologous d-AATs ... (Atasp1,accession number AY059912; Atasp3, AY050765; Atasp5,AY054660); putative d-AAT (AY099783); and < /b> BCAT (Atb-cat2, AY370135; Atbcat4, AY052676).Growth conditions of < /b> A. thaliana and < /b> thepreparation of < /b> its...
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