Báo cáo khoa học: " In vivo assessment of catheter positioning accuracy and prolonged irradiation time on liver tolerance dose after single-fraction 192 Ir high-dose-rate brachytherapy" pot

... et al.: In vivo assessment of catheter positioning accuracy and prolonged irradiation time on liver tolerance dose after single-fraction 192 Ir high -dose- rate brachytherapy. RadiationOncology ... catheter contribution indices indicated a lower tolerance dose of the liver parenchyma in areas with prolonged irradiation (p < 0.005).Conclusions: Positioning accuracy of brachytherapy catheters ... Discussion In this study, we sought to assess two methodicalaspects of HDR-BT: first, limits of catheter positioning accuracy and, second, effe cts of prolonged irradiation on the tolerance dose of...

Báo cáo khoa học: In vivo production of catalase containing haem analogues pot

... essentiallyinactive, and that this is not due to inactivation duringisolation of the protein.Light absorption spectra of the puriﬁed iron-con-taining and gallium-containing catalases are presented in ... gallium, tin, and zinc, and also variants con-taining meso-protoheme IX, ruthenium meso-protoporphyrin IX and (metal-free) protoporphyrin IX. Engineered haem proteins of this type are of potential ... catalase proteinscontaining Fe-meso and Ru-meso. Supplementation of the haem-free growth medium with PP resulted, muchto our surprise, in PP-containing catalase, i.e. proteincontaining a metal-free...

Báo cáo khoa học: In vivo studies of altered expression patterns of p53 and proliferative control genes in chronic vitamin A deﬁciency and hypervitaminosis pot

... analysis of c-jun,p53 and p21WAF1/CIF1 in liver and lung of control, chronic vitamin A-deﬁciency and hypervitaminosis ratsBased on the results found in the differential display study and taking into ... the present time. The role of vitamin A status in the expression of genesrelated to the regulation of cell proliferation has beenstudied in vivo and in vitro, and shows that retinoids play ... described in experimental proce-dures. A single band of about 53 kDa was detected in liver and lungindicating that the amount of p53 protein was signiﬁcantly increased in the liver and lung...

Báo cáo khoa học: In vivo degradation of nitric oxide synthase (NOS) and heat shock protein 90 (HSP90) by calpain is modulated by the formation of a NOS–HSP90 heterocomplex pot

... conditions of calpain activation, the protection of NOSdegradation occurs. In addition, the extent of NOS degradation is a func-tion of the level of HSP90 expression. Thus, in rat brain, which contains ... induced a mild elevation of intracellular Ca2+concentration ([Ca2+]i) by theadministration of a high-sodium diet (HSD) [28], and studied calpain degradation of NOS and HSP90 in brain and ... reproduced in in vitroconditions if nNOS digestion by calpain is carried out in the presence of HSP90 [27]. This ﬁnding can alsoexplain the large extent of digestion of eNOS in aorta, in which, in...

báo cáo khoa học: "In vivo observation of gold nanoparticles in the central nervous system of Blaberus discoidalis" potx

... effects of temperature, humid-ity, and wind were dim inished by mainta ining them i n acontrolled environment. However, the fluctuations in Figure 2 Nanoparticle injection site and direction is indicatedwith ... thelocation and direction shown in Figure 2, allowing toreach the brain of the insect. A stepper motor with speed and time control was used to inject 7 μLofnAuNPs/DIwater solution, giving 7 × ... reaction time and the amount of goldatoms present in the solution. This method delivers 95% of spherical particles and no further treatment was doneto eliminate the remaining 5% of non-spherical...

báo cáo khoa học: " In vivo reorganization of the actin cytoskeleton in leaves of Nicotiana tabacum L. transformed with plastin-GFP. Correlation with light-activated chloroplast responses" potx

... wortmannin were initially dissolved in DMSO and Disintegration of F-actin in EGTA and restoration of actin network prompted by calcium or magnesium ionsFigure 5Disintegration of F-actin in EGTA and ... file[http://www.biomedcentral.com/content/supplementary/1471-2229-9-64-S1.jpeg]Additional file 2Reorganization of F-actin during strong blue light irradiation. Changes in reorganization of actin bundles and in chloroplast distribution in lower mesophyll cells during SBL irradiation. ... cell after con-secutive irradiations with continuous SBL (20 min) and wBL (60 min). (A) Branched actin network in the dark-adapted cell; (B) Diffuse F-actin forming single widened strands after...

báo cáo khoa học: " In vivo imaging of the tonoplast intrinsic protein family in Arabidopsis roots" ppsx

... base of the elongation zone (panels A and F), TIP2;2-YFP and TIP2;3-YFP expression is firstobserved at the zone of transition with the differentiationzone (panels D and E). The onset of fluorescence ... two isoforms mirrors thepattern observed in single isoform localisation, withTIP4;1 expression being strongest in the epidermis and weaker in the cortex (Fig. 2F), and TIP2;3 expression beingstronger ... (reviewed in Frigerio etal, 2008). Recently we compared expression of TIP3;1 and TIP1;1 in Arabidopsis and found minimal overlap in thetiming of their expression, with TIP3;1 being abundant in embryos...

Báo cáo khoa học: " In vivo validation of the adequacy calculator for continuous renal replacement therapies" pps

... state of absence of information and of wide ignorance of the field, the calculator might have the merit of placing the issue of treatment dose among the priorities of critical care nephrology: a dose ... distinguish between different modalities. Parallel lines indicate stand-ard deviation. CVVH, continuous veno-venous hemofiltration; CVVHD, continuous veno-venous hemodialysis; CVVHDF, continuous ... average of 3.5 treat-ment days was examined for each patient. Nineteen post-dilution continuous veno-venous hemofiltrations (CVVHs), 23pre-dilution CVVHs, 23 CVVHDs and 41 post-dilution contin-uous...

Báo cáo khoa học: "A comparative study of Sephadex, glass wool and Percoll separation techniques on sperm quality and IVF results for cryopreserved bovine semen" pptx

... swelling test in predicting pregnancy rate and outcome in couples undergoing intrauterine insemination. J Androl 2002, 23, 498-502.44. Trentalance GM, Beorlegui NB. Sperm evaluation in cryopreserved ... solution (0.5 mg/mL in water) per mL of BO medium. CFDA/PI staining was carried out by incubating 100 μL of semen with 300 μL of staining media at 37oC for 15 min in the dark. A 5-μL aliquot of ... Shannon P, Curson B. Toxic effect and action of dead sperm on diluted bovine semen. J Dairy Sci 1972, 55, 614-620.40. Shen H, Ong C. Detection of oxidative DNA damage in human sperm and its...

Báo cáo khoa học: In vivo cross-linking of nucleosomal histones catalyzed by nuclear transglutaminase in starﬁsh sperm and its induction by egg jelly triggering the acrosome reaction pdf

... N-terminal region of core histones and by linker histone H1. Histones undergo avariety of post-translational modiﬁcations, including acety-lation, methylation, phosphorylation, ubiqutination, and ADP-ribosylation, ... CBB-stained actin on the gel.Ó FEBS 2003 In vivo cross-linking of histones (Eur. J. Biochem. 270) 3757 In vivo cross-linking of nucleosomal histones catalyzed by nucleartransglutaminase in starﬁsh ... cross-linking in vivo. Experimental proceduresCollection of spermSpecimens of A. pectinifera were collected, during theirbreeding season, from the coastal waters off Japan and weremaintained in...

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