... polymerase (Finnzymes) and the syntheticoligonucleotide primers 5¢-AAAAAAGAATTCATGTCAGACCTCAGCACCGC-3¢ and 5¢-AAAAAAAGCTTTCAGGCGGAACGCAGCTC-3¢ (EcoRI and HindIII restric-tion sites are underlined). ... Hisanaga Y, Ago H, Nakagawa N, Hamada K, Ida K,Yamamoto M, Hori T, Arii Y, Sugahara M, KuramitsuS et al. (2004) Structural basis of the substrate-specifictwo-step catalysis of long chain fatty ... by a lineargradient down to 5% buffer A in 2 min. 5% buffer A washeld for an additional 2 min and followed by a lineargradient up to 95% buffer A in 6 min.Determination of the acyl adenylate...