... [29–32].Taking these findings together, we suggest that the R69L mutant of IF1 allows premature subunit joiningby either failing to discriminate between certain TIRelements in mRNA, or inducing a conformation ... pKK3535 was the sole source of rRNA in either SQZ10 or JB69, asmuch as 59% of the termini were in the fully matureform, with the )7 and, to a lesser extent, the )3 spe-cies accounting for the rest. ... °Cto23°C during cul-turing before examining the ribosome profiles. During the course of these experiments, we noted that, upondownshift to the nonpermissive temperature of 23 °C,there were clear...