... and PE-conjugatedIgG2a (Becton Dickinson) and FITC-conjugated anti-CD4 and PE-conjugated anti-CD3 (Becton Dickinson) wereused as negative and positive controls, respectively. Flowcytometry ... RL,Weyand CM: Monoclonal T-cell proliferation and plaque insta-bility in acute coronary syndromes. Circulation 2000, 101:2883-2888.7. Markovic-Plese S, Cortese I, Wandinger KP, McFarland HF, ... (300,000–500,000)were stained with fluorescein isothiocyanate (FITC)-conju-gated anti-CD4 and phycoerythrin (PE)-conjugated anti-CD28 monoclonal antibodies (Becton Dickinson, SanJose, CA, USA). FITC-conjugated...