... to day 3 in the
spleen. Increases of the corresponding proteins were significant
in comparison with day 0 only in the case of IL-2 (day 6). By day
6 (transition to the chronic phase), the mRNA ... for any cytokine in any
organ at any time point.
Discussion
The present study documents that, in AIA, elevation of IL-
1β in the SM as well as elevations of IL-...
... organs.
9
The purpose of this study was to evaluate the
safety and efficacy of oral Apatone administered
throughout the day in prostate cancer in patients who
failed standard therapy.
Int. J. ... months and 11 continued for
at least 1 year with one patient continuing for more
than 2 years. Therapy was not discontinued in any
patient due to vitamin toxicity or for...
... only the joint -of- interest in Exercise I; in the
remaining six cases, the two independent readers did not
score repair in the joint -of- interest, which was in agreement
with the judgement of the ... constrained by the
number of joints with damage, and probably also by the level
of damage in those joints. In fact, data from animal studies
clearly indic...
... illu-
mination limited to 50 lux for the duration of their time
in the laboratory, from 4.7 to 5.6 days. They were not per-
mitted in distant parts of the laboratory near windows or
daylight. During ... it was the actual offset of synthesis of
melatonin in the pineal, controlled by the suprachias-
matic nucleus circadian system, which became relatively
delayed in...
... be
quickly separated from the bulk of other bacterial
proteins by using metal chelate affinity chromatog-
raphy [11]. Because N-terminal His-tag may influence
the function of protein, we designed ...
L-arginine, 10% glycerin) at 4°C. The buffer was
changed every 12h. The protein concentration was
measured by BCA Protein Assay Kit. PEG20000 was
used to concentrate the...
... omission of the
primary or the secondary antibody or primary incubation in the presence of non-immunized rabbit
serum instead of the primary antibody.
Cell quantification and Statistical analysis ... hPMS2 and GTBP/hMSH6 are very important in
distinguishing and repairing misparing and slippage errors in DNA synthesis. The mismatch is detected
by two complexe...
... protein as judged by SDS/
PAGE. The activity of the pure recombinant protein was
80% of that of t he wild-type protein in both the lysozyme
and chitinase assays. Attempts to further purify the
recombinant ... to the formation of the
oxazolinium intermediate in concert with Asp125, but not
to the binding of the substrate.
Comparison of our kinetic data w...
... coding for the subunits A and B of
V-ATPase. The intracellular localization of these isoforms
and the combination of the isoforms is yet to be determined.
We are now trying to express fusion proteins ... [10]. The YN11 strain was derived from
YPH499 strain, and YN45 strain was prepared by the use of
YN11 and BJ5458 strains.
Preparation of
AACEVAPD1 –6
5
0
RACE a...
... on the expected molecular size of the polypep-
tides, all the proteins of interest were present in the soluble
fraction of the induced cell in the case of the expression of
pET22b(+)/touBEA and ... mass of about 10, 38 and 57 kDa, the
expected molecular size of recombinant subunits B, E and
A, respectively. The identity of the proteins was further...
... substrates by the Conus
carboxylase. The propeptide of these substrates binds tightly
to the carboxylase and, as with the bovine carboxylase,
represents all or almost all of the binding energy for ... with
MEGALIGN
program), and 65% are conserved using
BLAST
analysis
comparing all of the vitamin K-dependent carboxylases.
In contrast, the C-terminal 25% of the pro...