... higher concentrations of GdnHCl.Autoproteolysis is independent of the proteinconcentration up to 5 m GdnHCl, as concluded fromdegradation kinetics between 1 and 100 lgÆmL)1pWT(Fig. 4). Hence, ... 405, 241–244.24 Veltman OR, Vriend G, Berendsen HJ, Van den BurgB, Venema G & Eijsink VG (1998) A single calciumbinding site is crucial for the calcium-dependent thermalstability of thermolysin-like ... nondegraded protein was quantified asdescribed in the Experimental procedures. Each value is the aver-age from three independent experiments.Engineered disul de bridge mimics calcium effect P....