Báo cáo khoa học: "The conformation change of Bcl-2 is involved in arsenic trioxide-induced apoptosis and inhibition of proliferation in SGC7901 human gastric cancer cells" doc

... Zheng et al., The conformation change of Bcl-2 is involved in arsenic trioxide-induced apoptosis and inhibition of proliferation in SGC7901 human gastric cancer cells World Journal of Surgical Oncology ... in any medium, provided the original work is properly cited.ResearchThe conformation change of Bcl-2 is involved in arsenic trioxide-induced apoptosis and inhibition of proliferation in SGC7901 ... at determining whether arsenic trioxide inhibits proliferation and induces apoptosis in SGC7901 human gastric cancer cells, accompanied by conformational changes of Bcl-2 and changes in total...

Báo cáo khoa học: The Vps4 C-terminal helix is a critical determinant for assembly and ATPase activity and has elements conserved in other members of the meiotic clade of AAA ATPases pdf

... thehomotypic interaction and the dominant-negativeeffects of Vps4p–E233Q–RDF in vivo. By contrast, loss of the b domain, which abolishes Vta1p binding,would abolish Vps4p oligomerization in vivo and in ... sequence in modulating the function of the SRH.The C-terminal region of human VPS4B containsthe b domain (b strands 7 and 8), the ﬁnal helix of theAAA domain (a helix 10) and the C-terminal helix(a ... mutant proteinstested retained the ability to interact with a panel of known Vps4p interactors indicating that the N-terminaldomain is correctly folded in each of the mutantproteins and that...

Tài liệu Báo cáo khoa học: The splicing factor ASF/SF2 is associated with TIA-1-related/ TIA-1-containing ribonucleoproteic complexes and contributes to post-transcriptional repression of gene expression doc

... in thepresence of RNAse. Interestingly, most of the identi-ﬁed proteins are known to be involved in RNA metab-olism and belong to three large families of RNA-binding proteins. These proteins ... domains involved in protein–proteininteractions and sub-cellular targeting [3,4]. TIA-1-related (TIAR) protein belongs to the RNA recogni-tion motif (RRM) family of RNA-binding-proteins. It is ... theabundance of each ARE-BP [53]. Interestingly, thedown-regulating effect of ASF ⁄ SF2 is preserved uponinactivation of its RNA-binding ability by point muta-tions in both RRM RNA-binding motifs...

Tài liệu Báo cáo khóa học: The unusual methanogenic seryl-tRNA synthetase recognizes tRNASer species from all three kingdoms of life pptx

... On the other hand, it is diminished by refolding of tRNA in 10 mMTris/HClpH 7.0 containing 5–10 mMmagnesium. However, theratio of monomeric and dimeric fractions after refoldingdepends on ... organelles, is oftenmissing in the sequences of methanogenic tRNAsSer.Thesame is with base 20B, which is characteristic for bacterial and organellar tRNASer. The role of D-loop insertion at20B in ... wasshown by aminoacylation and gel mobility shift assays. Thisindicates the conservation of some serine determinants in allthree domains and gives additional support to the existence of a functional...

Tài liệu Báo cáo khoa học: The SCO2299 gene from Streptomyces coelicolor A3(2) encodes a bifunctional enzyme consisting of an RNase H domain and an acid phosphatase domain pdf

... of Nocardia farcinica, and Tfu,Tfus02000308 of Thermobiﬁda fusca.TheRv2228c of M. tuberculosis is identical toMb2253c of Mycobacterium bovis.N. Ohtani et al. A fusion protein consisting of ... recombinant protein accu-mulated intracellularly in a soluble form. On the otherhand, when induced at 37 °C, almost all of the proteinaccumulated in an insoluble form. Recombinant pro-teins of ... examined here is a bifunctional enzyme con-sisting of an RNase H domain and an APase domain, and it is a novel style in the Type 1 RNase H family.Experimental proceduresCells, plasmids, and...

Báo cáo khoa học: The Mycobacterium tuberculosis ORF Rv0654 encodes a carotenoid oxygenase mediating central and excentric cleavage of conventional and aromatic carotenoids doc

... functions, mainly as precursors of retinoids[e.g. retinal and vitamin A (retinol)] [10–12]. Retinalconstitutes the visual chromophore of rhodopsins [13],whereas vitamin A and its derivative retinoic ... tuberculosis, the causative agent of tuberculosis, is an intracellular human parasite infect-ing approximately two billion people and causing ninemillion new cases of tuberculosis and approximatelytwo ... Bwithin 1 min, then increasing the ﬂow rate to 2 mLÆmin)1within 1 min and maintaining these ﬁnal conditions foranother 14 min.To determine the relative ratios of the C18- and C20-prod-ucts,...

Báo cáo khoa học: The twin-arginine translocation (Tat) systems from Bacillus subtilis display a conserved mode of complex organization and similar substrate recognition requirements doc

... contain differing numbers of TatC-con-taining domains and this important question meritsfurther attention.The most notable characteristic of the TatAd com-plex is that it displays none of the ... results in a complete block in export, as no mature-sized GFPband is evident in the periplasmic sample (P). Substitu-tion of single arginine residues to lysine (RK, and KR)results in a level of ... Aslower running band in lanes corresponding to the TatAy elution is indicated with an asterisk. (C) Streptactin column ﬂow-through and wash fractions containing free TatAy complexes isolated from...

Báo cáo khoa học: An E3 ubiquitin ligase, Synoviolin, is involved in the degradation of immature nicastrin, and regulates the production of amyloid b-protein doc

... Synoviolin increases the mem-brane localization of huntingtin protein [18], also sug-gesting that Synoviolin is involved in intracellulartrafﬁcking.We also found that NCT interacts with Synoviolin(Fig. ... functional deletion of Synoviolin causes a change in the intracellular trafﬁcking of NCT.Effect of Synoviolin on the production of AbNCT is one of the essential cofactors of the c-secretasecomplex. ... Synoviolin, strongly suggesting that nicastrin is the substrate of Synoviolin. Interestingly, amyloid b-protein generation was increased bythe overexpression of Synoviolin, although the nicastrin...

Báo cáo khoa học: "The Merendino procedure following preoperative imatinib mesylate for locally advanced gastrointestinal stromal tumor of the esophagogastric junction" ppsx

... car-diospasm. In its final version, the operation consists of theinterposition of an isoperistaltic segment of jejunumbetween the esophagus and stomach, with bilateral vagot-omy and a pyloroplasty. In ... metastatic GIST [8]. The therapy is well toler-ated with mild side effects diminishing with continuoustreatment.Although GIST can arise everywhere in the gastrointestinaltract, they most often ... publishing this case reportReferences1. Coindre JM, Emile JF, Monges G, Ranchere-Vince D, Scoazec JY: Gas-trointestinal stromal tumors: definition, histological, immu-nohistochemical, and...

báo cáo khoa học: "The behaviour change wheel: A new method for characterising and designing behaviour change interventions" pps

... range of mechan-isms that may be involved in change, including thosethat are internal (psychological and physical) and thosethat involve changes to the external environ ment. In general, insufficient ... to service provisionProducing and disseminating treatment protocolsFiscal Using the tax system to reduce or increase the financial cost Increasing duty or increasing anti-smuggling activitiesRegulation ... successfulbehaviour change interventions. This requires an appropriate method for characterising interventions and linkingthem to an analysis of the targeted behaviour. There exists a plethora of frameworks of...

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