...
the
atmosphere
and
the
intercellular
system
inside.
Intercellular
spaces
1-5
pm
in
diameter
are
frequently
found
in
the
lenticel
tissue
of
young
leaf-
less
alders
(K6stler ...
may,
according
to
the
theory,
be
due
to
the
existence
of
small
intercellular
spaces
inside
the
lenticel
tissue
stimulating
ther-
mo-osmosis
of
gases.
In
A.
incan...
... analysis by the
amplification of housekeeping gene GAPDH as described
previously [25]. Mouse TPH was amplified by a single PCR,
while serotonin AANAT was amplified by nested PCR. The
localization of ... Memphis, TN, USA;
4
Department of Internal Medicine, Southern Illinois University, Springfield, IL, USA
We showed expression of the tryptophan hydroxylase gene
and of tryptophan hy...
... superfamily play import-
ant roles in the inhibition of serine proteases involved in complex systems.
This is evident in the regulation of coagulation serine proteases, especially
the central ... (RCL) of the serpin [5]. The catalysis of the
peptide bond cleavage appears to be arrested at the
acyl intermediate by the unique action of the serpin,
whereby the...
... identified the pres-
ence of P- LAP protein in the renal distal tubules and col-
lecting ducts. In rat NRK52E cells derived from renal
tubules, P- LAP was localized mainly in the intracellular
compartment. ... placental leucine
aminopeptidase; vasopressin.
The serum level of placental leucine aminopeptidase
(P- LAP)/oxytocinase increases during pregnancy [1]. It is
general...
... chimeric protein
All the chimeric proteins were purified following the one-
step procedure reported previously for the purification of
horse recombinant PLRP2 expressed in insect cells [8]. The
culture ... composed
of a PL domain and a PLRP2 domain are described in
Fig. 1. First, pVLhoPL, previously described, resulted in
the integration of the nucleotide sequence encodi...
... the isoleucine residue is the most important:
even its replacement by a very closely related amino
acid, leucine, substantially impairs the intracellular
phosphorylation of 4E-BP1. Proline also ... The level of phosphorylation of Ser64 in
insulin-treated cells is therefore especially informative.
We have shown previously that mutation of Phe113
to alanine in the 4E-BP1 T...
... and
0.5 mL of 6 m HCl was added to the lyophilized protein in
sealed glass vials. The vials were incubated in the dark for
16 h at 95 °C and lyophilized. Samples were resuspended in
1 mL of deionized ... determination of the peptide–flavin complex
was carried out simultaneously with the chromatogra-
phy steps using an LC-MS ⁄ MS setup (see Experimen-
tal procedures). Flav...
... hydrolase; MCA, middle cerebral artery; MCAo, middle cerebral artery occlusion; NAPE, N-acylphosphatidyl-
ethanolamine; NAPE-PLD,N-acylphosphatidylethanolamine-hydrolyzing phospholipase D; NArPE, ... stages of reperfusion in the
ischemic striatum. Striatal downregulation of FAAH
and upregulation of NAPE-PLD activity lead to
increased levels of AEA, which in turn may play a role...
... subsequently analyzed in six wells, each in
a thin-walled 96-well PCR plate in 50 lL aliquots per well.
The real-time PCR machine used was an i-Cycler (Bio-Rad,
Hercules, CA, USA) providing a MyiQ Optics ... on lipophilic substrates, and the open form of
the loop interacts with the hydrophobic part of choles-
terol and is thus responsible for substrate specificity
[34]. Simil...
... (Smo), and Gli1 genes of Hh signaling were
significantly upregulated in CML patients when compared with normal people (P < 0.001). The levels of Shh, Smo
mRNA in chronic phase of CML patients ... receptors in CML patients and normal control. Lane 1:normal control 1:Lane 2:normal control 2:Lane 3:
CML-CP case 1:Lane 4:CML-CP case 2:Lane 5:CML-AP case 1:Lane 6:CML-AP case 2:La...