Báo cáo khoa học: "The role of photoperiod and temperature in the induction and the release of dormancy in Pinus sylvestris L seedlings" potx
Tài liệu Báo cáo khoa học: A role of miR-27 in the regulation of adipogenesis ppt
... IDM (10 lgÆmL)1insulin, 1 lm dexamethasone, and 0.5 mm IDM). Cells were then maintained in DMEM con-taining 10% fetal bovine serum and 1 lgÆmL)1insulin. The medium was replaced every other ... biologicallyrelevant role of miR-27 in the regulation of adipose tis-sue functions in vivo, we examined the expression of miR-27 in the genetically obese ob ⁄ ob mice. The expression levels of ... expression levels were determined in 3T3 -L1 cells at the protein level using western blot analysis onday 1 and day 4 of differentiation, a time frame forobservation of early genes and induction of...
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Tài liệu Báo cáo khoa học: A role for the intersubunit disulfides of seminal RNase in the mechanism of its antitumor action docx
... lgÆmL)1 of radioactively labelled125I-labelled MSSAE in binding buffer(DMEM containing 1 mgÆmL)1BSA and 25 mMHepes atpH 7.5). At increasing time intervals, cells were washedrepeatedly with NaCl/Piandthentreatedwith0.6MNaCl in ... by linking the exposedsulfhydryls to small thiols through formation of mixeddisulfides. We found that sulfhydryls from cell monolayers and cell membranes can attack these mixed disulfides in the monomeric ... residuesoriginally involved in the intersubunit disulfide bonding of BS-RNase, are irreversibly blocked through S-alkylation. In the active MSSAE and MSSG monomers, instead, the two Cys residues still form...
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Tài liệu Báo cáo khoa học: Key role of the loop connecting the two beta strands of mussel defensin in its antimicrobial activity docx
... results obtained by other groupswho also point to the role of the connecting loop of the b-hairpin of defensins. A combination of mutationalanalysis [35] and structural analysis of the plant ... infer whether the key role of the b-hairpin loop of MGD1 (loop 3) in antimicrobial activity of MGD1 is of general value in the mechanism of action of defensins. However, our resultscould be compared ... in which the sequence of the b-strands was maintained but the amino acids from loop 3 had been replaced bymultiple Ser and Gly residues. Therefore, in the sequence of the whole b-hairpin structure of...
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Tài liệu Báo cáo khoa học: Electrostatic role of aromatic ring stacking in the pH-sensitive modulation of a chymotrypsin-type serine protease, Achromobacter protease I pdf
... [10]. Further interest in the aromaticstacking is in the role of the electrostatic properties in enzymatic catalysis of API, and in distinguishing the functionally catalytic quadruple Ser194–His57–Asp113–His210 ... remainedparallel with the side-chain of His210. On the other hand, asmall side-chain at position 169, typically W169V and W169A, deviates from the original position. In the struc-tural deviation, ... chymotrypsin and bovine trypsin. The catalytic triadresidues and the substrate binding S1 pocket are locatedincloseproximitytotheactivesite.Thestructuralalignment of the catalytic triad residues and...
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Báo cáo khoa học: Caenorhabditis elegans metallothionein isoform specificity – metal binding abilities and the role of histidine in CeMT1 and CeMT2 potx
... and specialization events throughtheir evolution. In an attempt to relate MT functionalperformance at the molecular level (metal-bindingabilities) and the role of MT at the physiological level(metabolic ... Thus, the overall availableinformation suggests that: (a) C. elegans MTs aremost likely involved in basic biological processes and (b) the role of CeMT1 in global metabolism is morecritical than ... is markedly clearer towards Zn forCeMT1. These metal-binding features are in full con-cordance with an involvement of CeMT1 in the globalmetabolism of physiological Zn, as well as the contri-bution...
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Báo cáo khoa học: Critical role of the plasma membrane for expression of mammalian mitochondrial side chain cleavage activity in yeast pptx
... for cholesterol in adrenal cells ([40,54]). In addition to normally cycling between the PM and othercellular organelles, free ergosta-5-eneol also must be avail-able in the yeast cell to serve ... results in an increased level of free sterol (Fig. 4B), and it would also be expected to increase the cycling of sterols by esterification and hydrolysis, resulting in anincrease in intracellular ... Thus, the two estersynthases, Arelp and Are2p and the probable steryl esterhydrolase Tgllp, play complementary roles in maintainingfree ergosta-5-enol at appropriate levels, reminiscent of the mechanism...
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Báo cáo khoa học: Essential role of the C-terminus in Melanocarpus albomyces laccase for enzyme production, catalytic properties and structure pdf
... reesei )in lanes 1 and 7, ScMaL pool I, containing the more heavily overgly-cosylated laccase, in lanes 2 and 5, and the less overglycosylatedlaccase ScMaL pool II in lanes 3 and 6.Function of ... the folding of the protein during the post-translational processing, and thereby the function of the mature laccase.Our structural analysis of wild-type MaL indicatedthat the last four amino ... wild-type ScMaL and the Sc(delDSGL559) and Sc (L5 59A) mutants. The CD spectra of wild-type ScMaL (solidline) and the Sc(delDSGL559) (dotted line) and Sc (L5 59A) (doubledotted line) mutants...
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Báo cáo khoa học: Dual role of Nbs1 in the ataxia telangiectasia mutateddependent DNA damage response ppt
... formation of MRNfoci [19–21]. The Mre11-binding domain of human Nbs1 hasbeen localized to amino acids 682–693 in the COOH-terminal region of the protein. Deletion of this region of Nbs1 results in ... contains an intact Mre11-bindingdomain. Differences in the nature of the Nbs1 muta-tion, as well as in the dose of radiation administeredto NBS cells, thus probably underlie, at least in part, the ... domain in itsNH2-terminal region, as well as an Mre11-bindingdomain and an ATM-binding domain in its COOH-terminal region (Fig. 1). FHA and BRCT domains areoften present in eukaryotic nuclear...
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Báo cáo khoa học: Functional role of the linker region in purified human P-glycoprotein pot
... hydrolysis and transport are proba-bly uncoupled by cleavage of the linker [22]. Althoughthese results provided valuable suggestions for the role of the P-gp linker region, the molecular details, ... kinetic analy-ses of the native and linker-cleaved P-gp. In the present study, we investigated the functional role of the linker region of human P-gp using a highlypurified and properly folded ... This kinetic analysis provided new insightinto the role of the linker region. In addition, we fur-ther analyzed the functional role of the linker regionbased on the crystal structures of a...
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Báo cáo khoa học: Functional role of Bb-chain N-terminal fragment in the fibrin polymerization process pdf
... (TPBS), and 100 lL of mAbs solutions in NaCl ⁄ Piwere added to the wells and incubated for 60 minat 37 °C. After washing of the plate, 100 lL aliquots of a1 : 1000 solution in TPBS of the rabbit ... & Medved L (2002) Interaction of fibrin(ogen) with the endothelial cell receptorVE-cadherin: mapping of the receptor-binding site in the NH2-terminal portions of the fibrin beta chains.Biochemistry ... model the intermolecular binding between the D-domain of one fibrin desAA molecule and Bb12–46 of another(Fig. 8). The model was prepared with the pymolMolecular Graphics System [24] on the...
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Từ khóa: Nghiên cứu sự biến đổi một số cytokin ở bệnh nhân xơ cứng bì hệ thốngNghiên cứu vật liệu biến hóa (metamaterials) hấp thụ sóng điện tử ở vùng tần số THzđề thi thử THPTQG 2019 toán THPT chuyên thái bình lần 2 có lời giảiGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitĐỒ ÁN NGHIÊN CỨU CÔNG NGHỆ KẾT NỐI VÔ TUYẾN CỰ LY XA, CÔNG SUẤT THẤP LPWANPhát triển mạng lưới kinh doanh nước sạch tại công ty TNHH một thành viên kinh doanh nước sạch quảng ninhTrả hồ sơ điều tra bổ sung đối với các tội xâm phạm sở hữu có tính chất chiếm đoạt theo pháp luật Tố tụng hình sự Việt Nam từ thực tiễn thành phố Hồ Chí Minh (Luận văn thạc sĩ)Phát hiện xâm nhập dựa trên thuật toán k meansNghiên cứu về mô hình thống kê học sâu và ứng dụng trong nhận dạng chữ viết tay hạn chếNghiên cứu tổng hợp các oxit hỗn hợp kích thƣớc nanomet ce 0 75 zr0 25o2 , ce 0 5 zr0 5o2 và khảo sát hoạt tính quang xúc tác của chúngĐịnh tội danh từ thực tiễn huyện Cần Giuộc, tỉnh Long An (Luận văn thạc sĩ)Thiết kế và chế tạo mô hình biến tần (inverter) cho máy điều hòa không khíSở hữu ruộng đất và kinh tế nông nghiệp châu ôn (lạng sơn) nửa đầu thế kỷ XIXKiểm sát việc giải quyết tố giác, tin báo về tội phạm và kiến nghị khởi tố theo pháp luật tố tụng hình sự Việt Nam từ thực tiễn tỉnh Bình Định (Luận văn thạc sĩ)Giáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtChiến lược marketing tại ngân hàng Agribank chi nhánh Sài Gòn từ 2013-2015MÔN TRUYỀN THÔNG MARKETING TÍCH HỢP