Báo cáo y học: "Attenuation of inflammatory polyarthritis in TNF transgenic mice by diacerein: comparative analysis with dexamethasone, methotrexate and anti-TNF protocols" docx

... articleAttenuation of inflammatory polyarthritis in TNF transgenic mice by diacerein: comparative analysis with dexamethasone, methotrexate and anti -TNF protocolsEleni Douni1, Petros P Sfikakis2, Sylva ... efficacy of diacerein in preventing thedevelopment of pathology in the Tg197 transgenic murinemodel of RA, in which inflammatory polyarthritis is clinicallydetectable at 4 weeks of age, with ... synovial hypertrophy (<5 cell layers) with few inflammatory cells; 2 = moderate synovial hyper-trophy (< 20 cell layers) with accumulation of inflammatory cells into intrasynovial cysts; 3...

Báo cáo Y học: Inhibition of hyaluronan synthesis in Streptococcus equi FM100 by 4-methylumbelliferone doc

... MU-meta-bolites in the inhibition of HA synthesis by MU in FM100cells. These above results indicate that something requiredfor a fully functional HA synthesis system is down-regulatedor inhibited in intact ... Furtherstudy will be required to conﬁrm this interpretation and tounderstand fully the mechanisms for inhibition of HAsynthesis by MU. This information may be useful in thetreatment of diseases involving ... the culture medium was quantitated by asandwich binding protein assay using a very speciﬁc HABP(Fig. 3). By 8 h of culturing, a small amount of HA releasedinto the culture medium was observed,...

Báo cáo Y học: Interaction of bovine coagulation factor X and its glutamic-acid-containing fragments with phospholipid membranes A surface plasmon resonance study pdf

... Ca2+ligatingamino acids in the N-terminal part of the ﬁrst EGF-likedomain of factor X in uences the amidolytic activity of theintact protein [36]. However, direct interactions between theGla and serine ... for binding intact factor X and Gla–EGFN(data not shown).Kinetics of membrane bindingThe kinetics of binding to PL membranes of the zymogenfactor X, activated factor X (factor Xa) and the ... Mode of action of vitaminK and calcium binding properties of bovine prothrombin. Bio-chemistry 11, 4961–4964.24. Stenﬂo, J. & Ganot, P. (1973) Binding of Ca2+to normal and dicoumarol-induced...

Báo cáo y học: "Alterations of metabolic activity in human osteoarthritic osteoblasts by lipid" pdf

... 3) determination, respectively. The effect of HNE combined with TNF- α was evaluated by incubating osteoblasts with HNE (20 μM) for 30 minutes and subsequently stimulating them with TNF- α (1 ... producing excess bone-resorbing cytokines and prostaglandins[11]. Among the pro- inflammatory mediators, interleukin-6 (IL-6) is a multifunctionalcytokine involved in osteoclast recruitment and ... activity. We next examined the effects of HNE on the signaling pathways controlling cyclooxygenase-2(COX-2) and interleukin-6 (IL-6) expression in view of theirputative role in OA pathophysiology....

Báo cáo y học: "volution of an adenocarcinoma in response to selection by targeted kinase inhibitors" potx

... analysis of theprotein coding regions was also able to determine thatthe drug binding sites for sunitinib were intact. Clearly,many other changes have occurred within the tumorthat likely ... betadynein heavy chain 7) (Ciliarydynein heavy chain 7)(Dynein heavy chain-likeprotein 2) (HDHC2)Recurrence 4 ENSG00000156234 CXCL13-00110639 78747983 G A R 56 R H C-X-C motif chemokine ... genes, TP53(D25 9Y) and a truncating mutation in RB1 (L234*)removing 75% of its coding sequence [24], with TP53also within a region of heterozygous loss (LOH).Transcriptome analysis Whole transcriptome...

Báo cáo y học: "Attenuation of murine antigen-induced arthritis by treatment with a decoy oligodeoxynucleotide inhibiting signal transducer and activator of transcription-1 (STAT-1)" ppt

... in a subgroup of patients with RA accompanied by a massive infiltration of inflammatory cells[8]. In addition, STAT-1 expression has been shown in T and B lymphocytes in focal inflammatory infiltrates, ... evalu-ation of arthritis (day 14 of AIA). Hematoxylin-eosin stained knee sec-tions were evaluated blindly by grading of inflammation (infl.; cell exudate or infiltrate, hyperplasia) and joint destruction ... treatment in synoviocytes and macrophages in vitroEfficacy of the STAT-1 decoy oligodeoxynucleotide (ODN) treatment in synoviocytes and macrophages in vitro. (a) Electrophoretic mobility shift analysis...

Báo cáo y học: "Expression of inflammatory host genes in Chlamydia trachomatis-infected human monocytes" docx

... IA, Quayle AJ, Visintin A, Ingalls RR: Local-ization of TLR2 and MyD88 to Chlamydia trachomatis inclu-sions. Evidence for signaling by intracellular TLR2 duringinfection with an obligate intracellular ... defensemechanisms involved not only in tuberculosis but also in ReA[18]. Interestingly, IL-12 was not differentially expressed in ourstudy, possibly accounting for the inability of the monocytes toeliminate ... upregulated in our study, possiblyindicating a typical chlamydial activation program of humanmonocytes.The highest ratios and numbers of cytokine genes are induced in the early, active infective...

Báo cáo y học: "Role of interleukin-7 in degenerative and inflammatory joint diseases" potx

... pathway. This is supported by recent findings demonstrating TNF -independent IL-7-driven inflammatory and bone-destructive activity [6,7].IL-7 is also able to regulate joint pathology by T cell-drivenimmune ... [9]. In addition,estrogen deficient mice (induced by ovariectomy) arecharacterized by increased IL-7-driven T cell-dependent boneloss [10]. By giving a first glimpse of the direct effects of ... increased bone volume and bone density [8]. In contrast, IL-7-overexpressing transgenic mice are character-ized by expanded bone marrow cavities with focal osteolysis of cortical bone and eroded bone...

Báo cáo y học: "Attenuation of fibrosis in vitro and in vivo with SPARC siRNA" ppsx

... reduced by their corresponding siRNAs(P = 0.002 and 0.0004, respectively).siRNAs of SPARC and Ctgf ameliorated fibrosis in skin and reduced inflammation in lungs induced by bleomycinHE stains of ... samples of both lung and skin were fixed in 4%formalin and embedded in paraffin. Sections of 5 μmwere stained either with hematoxylin and eosin (HE) and Masson's trichrome.Statistical analysis Results ... Four mice were used for each group. A. Injection with saline (negative control) only; B. Injection with bleomycin only; C. Injection with bleomycin and treatment with SPARC siRNA; D. Injection with...

Báo cáo y học: " Attenuation of antigen-induced airway hyperresponsiveness and inflammation in CXCR3 knockout mice" doc

... regulated by a variety of chemokines (5). The expression of distinct chemokine receptors on infiltrating cell populations, especially on lymphocytes and eosinophils which are highly implicated in ... other cytokines such as IL-13 are involved in the induction of IgE production in our model (32). We also showed that induction of mRNA expression of pro -inflammatory cytokine TNF in the lungs ... Changes in tracheal pressure were measured 19allergic airway inflammation via recruitment of CD8+ T cells into the airways to trigger the release of proinflammatory cytokines including TNF and...

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