... fluorometric HPLC utilizing the methoddescribed by Hara et al. [27]. The retention times of the282 Y. Shen et al. (Eur. J. Biochem. 271) Ó FEBS 2003because no correlation between O-acetylated ... MU, 4-methyl-umbelliferyl; 4-MUAc, 4-methylumbelliferyl acetate; Neu5Ac,5-N-acetyl-neuraminic acid; Neu5,9Ac2,5-N-acetyl-9-O-acetylneu-raminic acid; Neu5,7Ac2,5-N-acetyl-7-O-acetylneuraminic ... Australia.Fax: + 61 7 5552 8098, Tel .: + 61 7 5552 7029,E-mail: j.tiralongo@griffith.edu.auAbbreviations: AcCoA, acetyl-CoA; DMB, 1,2-diamino-4,5-methy-lenedioxybenzene; Kdn, 2-keto-3-deoxynononic...