... change in the conformation of this loop. Changes in the conformation of the backbone at this point and changes in the orientation of other side chains, as aresult of the introduction of the large ... Full line, wild-type; short brokenlines, G376E; long broken lines, G157D. (B) Full line, wild-type; longbroken lines, wild-type in 0.3M NaClO4; short broken lines, W56F. The W56F sample contained ... activity rela-tive to the wild-type enolase. This is not surprising,considering the location of these changes. Glycine 157is in one of the loops that moves on binding of sub-strate and divalent...