Báo cáo khoa học: "Effect of edging and docking methods on volume and grade recoveries in the simulated production of flitches" pps
Báo cáo khoa học: "Effect of edging and docking methods on volume and grade recoveries in the simulated production of flitches" pps
... jthedger sawline and kth docking sawline;Original articleEffect of edging and docking methods on volume and grade recoveries in the simulated production of flitchesCL ... flitch. In the following extract, the term’trimming’ is equivalent to docking ; and ’cutting-line combinations’ refers to the com-binations of edging and docking lines." ... variation of cutting lines. Cutting line combinationswere generated by varying the coordinates of each edging and trimming line betweenpredetermined limits." These limits,...
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Báo cáo khoa học: Abortive translation caused by peptidyl-tRNA drop-off at NGG codons in the early coding region of mRNA potx
... inhibitgrowth of the strain even in the presence of IPTGinduction. All of these results suggest that the low geneexpression associated with NGG codons in the down-stream region following the initiation ... codon in the earlyregion downstream of the initiation codon. If the NGG codons in the downstream region are translatedslowly down to, and including position +5, this wouldgive extended pausing ... mechanism prone to a codondependent peptidyl-tRNA drop-off at the very begin-ning of the coding region in mRNA. In the case of NGG codons located close to the initiation codon suchinstability appears...
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Tài liệu Báo cáo khoa học: A second novel dye-linked L-proline dehydrogenase complex is present in the hyperthermophilic archaeon Pyrococcus horikoshii OT-3 pptx
... supported in part by the PioneeringResearch Project in Biotechnology of the Ministry of Agriculture, Forestry and Fisheries of Japan and by the National Project on Protein Structural and FunctionalAnalyses ... enzyme solutions were used as the purified enzyme preparations.Preparation of the P. horikoshii genomic DNA and cloning of the enzyme genesTo obtain the genomic DNA containing the PDH1 and PDH2 ... subunit of PDH2 and the b subunit of the T. profundus enzyme, respectively (Fig. 4). The b1 sub-unit of PDH1 contained an ADP-binding motif [6],which was well conserved in the b subunit of the...
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Báo cáo khoa học: Activated transglutaminase from Streptomyces mobaraensis is processed by a tripeptidyl aminopeptidase in the final step pptx
... [1]. In contrast, culture in shaking flasks containing aliquid complex medium prevents sporulation. The onset of aerial hyphae growth is closely associated with the secretion and activation of ... Ne-(c-glutamyl)lysine bonds, the incorpor-ation of polyamines into proteins, the deamidation of protein-bound glutamines, and the covalent attachment of proteins to lipids such as x-hydroxyceramides ... distinct differ-entiation stages. Culture on agar plates containing glucose,yeast and malt extracts allows the organism to developsubstrate and aerial mycelia culminating in the formation of spores...
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Báo cáo khoa học: "Effect of intern’s consecutive work hours on safety, medical education and professionalism"
... after 16 hours of sustainedwakefulness, a finding reflected in the twofold increase in interns’ attentional failures after they had been working formore than 16 hours on the traditional schedule ... total number of days in each schedule was the same. Under this assumption, the observation that there were more admissions and patient-days, yet the same number of interns, in the traditional armwould ... that interns in the intervention schedule “often knew very little about the patients who had been admitted the night before” and that the “intern coming on at 9 p.m. … had not considered the patient...
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Tài liệu Báo cáo khoa học: Effect of siRNA terminal mismatches on TRBP and Dicer binding and silencing efficacy pdf
... positionedafter the PAZ domain binds to one end of the duplex[36,41], thus the reduction in binding affinity may resultfrom the inability of the double-stranded RNA bindingdomain (dsRBD) to bind the disrupted ... siRNA, introducing a terminalmismatch that further reduces the stability of the guidestrand 5¢ end does not enhance the functionality of the siRNAs. Based on comparison of the binding and silencing ... relationship between the silencing efficiency of the siRNAs and theirbinding to the RNA-induced silencing complex loading complex proteinsHIV transactivating response RNA-binding protein and Dicer...
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Tài liệu Báo cáo khoa học: Effect of ionic strength and oxidation on the P-loop conformation of the protein tyrosine phosphatase-like phytase, PhyAsr docx
... with the cysteine Sc in the unoxidizedconformation. Oxidation of the catalytic cysteinedecreased the contacts made to the cysteine Scbutresulted in the formation of 17 additional interactionswith ... dictates whether the P-loop moves upon oxidation of the catalytic cysteine is the ability of the conserved active site arginine to moveEffect of ionic strength and oxidation on PhyAsr R. J. Gruninger ... ligand in the active site. Least squaressuperposition of the P-loop main chain (starting at the residue prior to the catalytic cysteine and ending after the conserved arginine) resulted in rmsd...
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Tài liệu Báo cáo khoa học: Effect of deletion of the DNase I hypersensitive sites on the transcription of chicken Ig-b gene and on the maintenance of active chromatin state in the Ig-b locus docx
... was introduced into DT40cells. Ig-b gene expression, acetylation of H3 and H4histones, and the remaining DHSs in the deleted cellswere examined to determine the roles of these DHSs on the ... genes, one at the transcriptional start site,one in the first intron of the Ig-b gene, four betweenIg-b and its downstream growth hormone (GH) genes, and one in the downstream region of the GH ... of the South-ern hybridization. Position of the probes, the size and position of restriction fragments hybridizable with the probe, and exon ⁄ introns areshown as in Fig. 2A. Positions of the...
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Tài liệu Báo cáo khoa học: Effect of sequence polymorphism and drug resistance on two HIV-1 Gag processing sites pdf
... 2).Increasing the concentration of the NC/p1 substrate above the Kmresulted in a decreased velocity, suggesting the possibility of increased nonproductive binding at the substrate binding site. Nevertheless, ... gene. Many of these mutations are located in the substrate binding site of the PR, and these mutationshave considerable impact on PR activity and specificity.Other resistant mutations alter residues ... residues are in bold.b The type of mutation: natural polymorphism (P), mutation appearing only in drug resistance (R, seeFig. 1). If two mutations occurred and one of them was occurring only in drug...
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Báo cáo khoa học: Effect of flanking bases on quadruplex stability and Watson–Crick duplex competition pptx
... obtained for complementary strand binding to the preformed quadruplexes at 25 °C. The quadru-plex concentration in the cell was 5–10 lM and the complementary strand concentration in the syringe ... of 0–12 bases in KCl. We observed enhance-ment of fluorescence intensity on increasing the con-centration of the complementary strand, indicative of agreater extent of quadruplex opening. The ... bases. The effect of loop length and composition on quadruplexconformation and stability has been well investigated in the past, but the effect of flanking bases on quadruplex stability and Watson–Crick...
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