... structure of the protein. Figure 2 shows the fluorescence spectra of wild-type SNase and the fourmutants E142O, K133A, W140A and W140O. The fluorescence spectra of E142O and K133A are similarto that of ... analysis of protein unfolding The DSC curves of the wild-type protein and the mutants K133A, E142O, W140A and W140O areshown in Fig. 3, with their thermodynamic parameterssummarized in Table 1. The ... FEBS of the wild-type protein and K133A and E142O were88.45, 84.70, and 83.60 kcalÆmol)1, respectively. The melting point and DSC profile of E142O (52.8 °C) wasalmost identical with that of the...