Báo cáo khoa học: "The article in possible status of wood quality oak breeding programs (Quercus petraea Liebl and Quercus robur L)" pps

... thattime.HOW SHOULD WOOD QUALITY BETAKEN INTO ACCOUNT IN A POSSIBLE OAK BREEDING PROGRAM?As described below, taking wood quality into account in an oak breeding programhas ... of wood quality for someforest species is sometimes discussed,these data clearly show that in oak, wood Original article The possible status of wood quality in oak breeding ... WOOD QUALITY INTO ACCOUNT IN AN OAK BREEDING PROGRAM?High level of phenotypic variabilityfor basic and technological properties in wood of oak All studies devoted to oak...

Báo cáo khóa học: The C-terminal t peptide of acetylcholinesterase forms an a helix that supports homomeric and heteromeric interactions potx

... catalytic domain of ratAChETor at the beginning of its t peptide, in the )5to6interval; in these mutants, the original cysteine was eitherretained or replaced by a serine (C37S). We also introducedcysteine ... hypothesis of a hairpin structure, an intracatenarydisulﬁde bond might be formed in mutants containing theoriginal cysteine or another C-terminal cysteine, togetherwith a cysteine in the N-terminal ... AChETsubunits, and betweenAChETsubunits and a PRAD-containing protein [theN-terminal fragment of the ColQ protein (QN)] possessingtwo cysteines upstream or downstream of the PRAD. Thisshows that, in...

Tài liệu Báo cáo khoa học: The role in the substrate speciﬁcity and catalysis of residues forming the substrate aglycone-binding site of a b-glycosidase docx

... resi-dues of the aglycone-binding site in the stabilization of ESà and the interdependence between the binding of aglycone and the positioning of glycone in subsite )1are not known. In this study, ... was included to facilitate the visualization of the active site. (C) Agly-cone-binding site of SbDhr1, including the substrate dhurrin. (D) Aglycone-binding site of BglB, including the inhibitor ... these datamay be of particular importance in understanding thephysiological role of b-glycosidases and in designinginhibitors. In addition, another important issue in understand-ing the aglycone...

Tài liệu Báo cáo khoa học: Phylogenetic relationships in class I of the superfamily of bacterial, fungal, and plant peroxidases pptx

... number of coding seq uences currently in the database may affect thisvalue. Interestingly, none of the known fungal genomicDNA sequences in which KatG is included (listed in Table 1) contain an intron, ... are offspring of this single gene transfer. It is also evident that, in acomplete fungal genome, two independent evolutionarylines of class I of this superfamily occur, i.e. the CCP line and ... set of 94 a nalysed peroxidase sequences. The bootstrapsupport in all main nodes is strong; only in some minornodes is reﬁning of the particular species within groupsmoderate. In the case of PUZZLE,...

Tài liệu Báo cáo khoa học: "The Thesaurus in Syntax and Semantics" docx

... policy of interlingualizing the thesaurus, (that is, of assimilating semantics to syntax) and that of thesaurizing the syntax (that is, of in- cluding syntax within semantics) the linguists ... rigorous separation of the particular from the comparative universal range of validity (in MT terminology, of monolingual from interlingual features), and by their separate handling in the program ... was rendered into English as 'the percentage of matured capsules and the number of grains of seeds of one capsule are different according to the time of hybridizing;' into German...

Báo cáo khoa học: The natural mutation by deletion of Lys9 in the thrombin A-chain affects the pKa value of catalytic residues, the overall enzyme’s stability and conformational transitions linked to Na+ binding pdf

... altering thegeometry and protonation state of the residues involved in catalysis and inhibitor binding, and (b) limiting theallosteric effects triggered by sodium binding.X-ray structures of ... withinthe A-chain and between the A- and B-chains occursmainly through salt bridges and H bonds involvingcharged side chains. The A-chain is intramolecularlycross-linked by ﬁve side-chain ... higher instability of the DK9 mutant compared with WT thrombin, and disulﬁde scramblingexperiments proved weakening of the interchain interactions, causing fasterrelease of the reduced A-chain in...

Báo cáo khoa học: The propeptide in the precursor form of carboxypeptidase Y ensures cooperative unfolding and the carbohydrate moiety exerts a protective effect against heat and pressure pot

... USA 88,9330–9334.6. Winther, J.R., Sørensen, P. & Kielland-Brandt, M.C. (1994)Refolding of a carboxypeptidase Y folding intermediate in vitroby low-aﬃnity binding of the proregion. J. Biol. ... thestepwise increase in temperature and pressure, the heat-induced unfolding of CPY and DglyCPYshowedatwo-state transition which is typical of a cooperative unfolding(Fig. 2B), but their pressure-induced ... anincrease in ANS-binding ﬂuorescence and a loss of enzymatic activity [25], indicating exposure of the hydro-phobic core to the solvent; further conformational changeinduced by higher pressures of...

Báo cáo khoa học: The N-terminal cysteine pair of yeast sulfhydryl oxidase Erv1p is essential for in vivo activity and interacts with the primary redox centre doc

... thecorrect arrangement of the FAD-binding fold. Variations in dimer formation and unique colour changes of mutantproteins argue in favour of an interaction between theN-terminal cysteine pair with the ... of the nine present in human Alrp and in addition, the mutatedyeast proteins can be easily checked for in vivo activity.Materials and methodsStrains and plasmidsThe following strains of S. ... modelling and the energy minimizing of theFig. 1. Comparison of Erv1p, DN-Erv1p and Erv2p. The ﬁgure displays the correspondingcysteine residues and identity values for theN- and the C-terminal...

Báo cáo khoa học: The multifarious short-term regulation of ammonium assimilation of Escherichia coli: dissection using an in silico replica pdf

... (PIIUMP3KG3), and of glutamine (GLN). The numbered lines in the insets of (A) and (B) correspond to the removal of thermodynamic regulation (1), kinetic regulation (2), and both (3). In order to ... between PIIKG1 and GLN. All kinetic data in Eqns (15a and b) (and Eqns 16c and d) resulted from ﬁtting the ATase kinetics with amodel containing the Eqns (1–4 and 7) to transient and steady-state ... models of parts of living cells, so-called silicon cells. These ‘silicon cells’ incorporate allexisting experimental information on the molecules of living cells into computer replica of parts of...

Báo cáo khoa học: The )148 to )124 region of c-jun interacts with a positive regulatory factor in rat liver and enhances transcription Dipali Sharma*, Sujata Ohri and Aparna Dixit ppt

... expression and activity are partly regulated by JunN-terminal kinases (JNKs) and mitogen activated proteinkinases. JNKs phosphorylate the N terminus of the trans-acting domain of Jun, thereby increasing ... binding protein that becomestotally inactivated within 15 min of heat treatment at 47 °C[34], junRP retains its DNA-binding activity, although at arelatively low level, even when the binding ... Detection and isolation of DNA-binding proteins using single pulse ultravioletlaser crosslinking. Anal. Biochem. 218, 248–254.26. Philippe, J. (1994) The Southwestern Assay. In Methods in Moleuclar...

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