... per-formed after cell extraction, or the need for derivatiza-tion. Cell disruption and saponification was performedas previously described, using a mixture of EtOH andKOH [20]. We found that using ... little effort made so far to engineer themetabolism of a microbial host for enhanced produc-tion of triterpenes. By contrast, there have been manyconsiderable efforts to engineer microbes for higher production ... Keasling1,3,4,51 California Institute for Quantitative Biomedical Research, University of California, Berkeley, CA, USA2 Department of Chemistry, University of California, Berkeley, CA, USA3...