... 5¢-GCCACTTACTTGAACTTTGACATAGAAGCCGG-3¢,5¢-GCCACTTACTTGGCATTTGACATAGAAGCC-3¢,5¢-GCCACTTACTTGGACTTTAACATAGAAGCCGG-3¢,5¢-GCCACTTACTTGGACTTTGCGATAGAAGCCGG-3¢,5¢-GGACTTTGACATACAAGCCGGTATCGATGC-3¢,5¢-GGACTTTGACATAGCGGCCGGTATCGATGC-3¢ and 5¢-GGATCACTAGCCTTCGCGAGTGTAGACAGAG-3¢, ... k-uegaki@aist.go.jpDatabaseStructural data are available at the ProteinData Bank under the accession numbers 3A4 W (E52 6A substrate complex), 3A 4X (D52 4A substrate complex) and 3AFB(D52 4A apo-form)(Received ... Osaka, Japan). Target primersfor the generation of D522N, D52 2A, D524N, D52 4A, E526Q, E52 6A and W66 4A mutations were 5¢-GCCACTTACTTGAACTTTGACATAGAAGCCGG-3¢,5¢-GCCACTTACTTGGCATTTGACATAGAAGCC-3¢,5¢-GCCACTTACTTGGACTTTAACATAGAAGCCGG-3¢,5¢-GCCACTTACTTGGACTTTGCGATAGAAGCCGG-3¢,5¢-GGACTTTGACATACAAGCCGGTATCGATGC-3¢,5¢-GGACTTTGACATAGCGGCCGGTATCGATGC-3¢...