... us ing 1 ng of labelled Jun-25 and 10 lg of nRNE-d (A) and rRNE-d ( B) in the presence of varying con-centrations of dist amyc in A (indicated ontop). (C,D) E ffect of actinomycin D (Act. D) and ... insensitivity of nRLjunRP and rRLjunRP to the major groove binding drug, m ethylgreen, coupled with its sensitivity to minor groove bindingdrugs, actinomycin D and distamycin A, confirms that theseare ... concen-trations of methyl green, a majo r groove b inding drug [41] and distamycin A, a minor groove binding drug [42].Increasing concentrations of distamycin A resulted in adecrease in the c omplex...