... binding to the high-affinity and low-affinitysites of tHSA–heme-Fe(III) and HSA–heme-Fe(III)[represented by a and (1 – a), respectively, in Eqn (1)]are 0.18 and 0.82, and 0.14 and 0.86, respectively(Table ... spectroscopic and catalytic properties of human serum heme–albuminAlessandra di Masi1, Francesca Gullotta2,3, Alessandro Bolli1, Gabriella Fanali4, Mauro Fasano4 and Paolo Ascenzi11 Department ... modulation of reactivity and spectroscopic properties; recombinanttruncated human serum heme–albumin(Asp1–Glu382)CorrespondenceP. Ascenzi, Department of Biology, and Interdepartmental Laboratory...