... for the
676 E. Barros et al.
E. Barros et al .Wood splitting markers in E. grandis
Note
Identification of PCR-based markers linked to wood splitting
in Eucalyptus grandis
Eugenia Barros
a*
, Steve ... limits the application of these markers to that
population. Here we report the identification of putative molecular markers linked to wood splitting...
... 1 min incubation in the presence of 5 l
M
L
-arginine as described in Experimental procedures.
L
-leucine (500 l
M
)
was added simultaneously to
L
-arginine. Valinomycin (10 l
M
)or
gramicidin ... trans-side of the membrane.
Moreover system y
+
L is inhibited by
L
-leucine in the
presence, but not in the absence of Na
+
.
The small inhibition of
L
-arginine in ux by
L
-leuc...
... within a
FORMULA in the Actor relation with respect to the head contain’]
contain( , )
CONTAINMENT(container , containee )
[’the Containment relation involves a predicate contain and its Actor
and ... of the intended proof-step.
Metonymy Metonymic expressions are used to
refer to structural sub-parts of formulae, resulting
in predicate structures acceptable informally, yet in-...
... selectivity of the palytoxin-induced pore
Assuming that palytoxin might induce cation-permeable
pores in the apical membrane, the Isc evoked by the toxin
should consist of an in ux of Na
+
, the ... sensitive to ouabain (g-strophan-
thine), the most well known inhibitor of the pump. Based on
these and other experiments showing the formation of
palytoxin-induced ion channels in...
... collected mainly in only one area (Ovar, in the north
of Portugal). This narrow genetic base and the danger of
severe inbreeding became a concern when afforestation
increased to provide in the growing ... pulpyield in provenances of Eucalyptus regnans, Appi-
ta 47 (1994) 159–162.
[19] Rodrigues J.C., Faix O., Pereira H., Determination of li-
gnin content of Eucalyptus...
... of
neck. In the middle of the trunk, two elliptical testes were
arranged linearly. Posterior to the testes, six cement glands,
pyriform in shape were arranged linearly, with ducts
leading into ... they were mounted in Canadian balsam.
In order to identify the worms, the length, and width of
the body and internal organs were measured. Species
identification was made accordi...
... protein specific to PEDV in porcine
enterocytes and about 66 kDa binding protein in ST cell.
The distinction of specific proteins of PEDV in enterocyte
and ST cells in size was supposed to allow ... to identify PEDV binding protein in
permissive cells. The binding ability of PEDV to porcine
APN (pAPN) and the effects of pAPN on infectivity of
PEDV in Vero cells w...
...
methanol
in
20
mM
triethyla-
mine
acetate
(pH
3.37)
to
65%
methanol
at
20
min
to
85%
methanol
at
25
min
to
100%
metha-
nol
at
27
min
holding
at
100%
until
40
min.
Data
were ...
as
an
indicator
of
date
of
lifting,
but
it
is
not
a
useful
indicator
of
plant
vigor.
Common
sense
suggests
that
plants
low
in
starch
might
have
difficult...
... sequence of the inser-
tion, PCR products corresponding to exon 4 were cloned
using the TOPO-TA Cloning Kit for Sequencing, (Invitro-
gen) according to manufacturer instructions. The product
of the ... the
present.
Cloning of the mutated exon 4
To further characterize the mutation, we cloned the
mutated PCR product into the pCR4-TOPO vector
(TOPO-TA Cloning Kit for Sequencing, Inv...